Purified thermostable Pyrococcus furiosus DNA polymerase I

Details Purified thermostable Pyrococcus furiosus DNA polymerase I Purified thermostable Pyrococcus furiosus DNA polymerase I

2006-05-16

2006-05-16

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S320100, C435S252300, C435S325000, C536S023200

Reexamination Certificate

active

07045328

ABSTRACT:
Purified thermostablePyrococcus furiosusDNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000–93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4994372 (1991-02-01), Tabor et al.
patent: 5210036 (1993-05-01), Comb et al.
patent: 5242818 (1993-09-01), Oshima et al.
patent: 5322785 (1994-06-01), Comb et al.
patent: 5352778 (1994-10-01), Comb et al.
Bernad, et al., “A Conserved3′-5′ Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases,”Cell, 59:219-228 (1989).
Berger, et al., “Guide to Molecular Cloning Techniques,”Meth. in Enzymol., 152:393-399; 415-423, 432-449, 661-704 (1987).
Blanco, et al., “Evidence favouring the hypothesis of a conserved 3′-5′ exonuclease active site in DNA-dependent DNA polymerases,”Gene, 112:139-144 (1992).
Bryant, et al.,J. Biol. Chem., 264:5070-5079 (1989).
Deutscher, “Guide to Protein Purification,”Meth. in Enzymol., 182:738-751 (1990).
Dutton, et al., “General method for amplifying regions of very high G+C content,”Nucl. Acids Res., 2953-2954 (1993).
Eckert, et al.,Nucl. Acids Res., 18:3739-3744 (1990).
Ennis, et al.,Proc Natl. Acad. Sci. USA, 87:2833-2837 (1990).
Forterre P.,Nucl. Acids. Res., 20(7):1811 (1992).
Hamal, Abdellah et al., “Purification and Characterization of a DNA polymerase from the ArchaebacteriumThermoplasma Acidophilum,” Eur. J. Biochem., 190(3):517-21 (1990).
Keohavong, et al.,Proc. Natl. Acad. Sci. USA, 86:9253-9257 (1989).
Kohler, et al.,Proc. Natl. Acad. Sci. USA, 88:7958-7962 (1991).
Klimczak, Leszek J., et al., “Purification and Characterization of DNA Polymerase from the ArchaebacteriumSulfolobus acidocalarius,” Nucleic Acids Research, 13(14):5269-82 (1985).
Klimczak, Lescek J., et al., “Purification and Characterization of DNA Polymerase from the ArchaebacteriumMethanobacterium thermoautotrophicum,” Biochemistry, 25:4850-55 (1986).
lawyer, F.C. et al. “Isolation Characterization and Expression inE. coliof the DNA Polymerase Gene FromThermus agriaticus,” J. Biol. Chem., 264(11): 6427-6437 (1989).
Lee, J.,Laboratory Methods, Alternative Dideoxy Sequencing of Double-Stranded DNA by Cyclic Reactions UsingTaq Polymerase, DNA and Cell Biology, 10(1):67-73 (1991).
Lundberg, et al., FASEBJ 5(6) A1549 (1991).
Lundberg KS, et al., “High-fidelity amplification using a thermostable DNA Polymerase isolated fromPyrococcus furiosus,” Gene, Dec. 1, 1991; 108(1):1-6.
Mathur, et al.,J. Cell. Biochem., 16B:29 (1992).
Mathur, et al.,Nuc. Acids. Res., 19(24):6952 (1991).
Perrino, et al.,Biochemistry, 29:5226-5231 (1990).
Reiss, et al.,Nucleic Acids Res., 18:973-978 (1990).
Rossi, M. et al, “Structure and Properties of Thermophilic and Thermostable DNA polymerase Isolated fromSulfolobus solfataricus,” System Appl. Microbiol, 7:337-341 (1986).
Ruttimann, Carmen et al., “DNA Polymerases from the Extremely Thermophilic Bacterium,”Thermus thermophilusHB-8,Eur. J. Biochem., 149 (1):41-46 (1985).
Saiki, et al.,Science, 239:487-491 (1988).
Simpson, H.D., et al., Purification of a Thermostable DNA Polymerase from aThermotogaSpecies,Ann. N.Y. Acad. Sci., 613:426-428 (1990).
Tindall, et al.,Biochemistry, 27:6008-6013 (1988).
Wahl, G. M. et al, “Molecular Hybridization of Immobilized Nucleic Acids: Theoretical Concepts and Practical Considerations,”Methods in Enzymology, 152:399-412 (1987).
Ward, John M., et al., “Phosphocellulose as a Tool for Rapid Purification of DNA-modifying Enzymes,”Analytica Chimica Acta, 249:195-200 (1990).
Wheelis, et al., “On the nature of global classification,”Proc. Natl. Acad. Sci., 89:2930-2934 (1992).

Affiliated with

Also associated with

Rating

Purified thermostable Pyrococcus furiosus DNA polymerase I does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified thermostable Pyrococcus furiosus DNA polymerase I, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified thermostable Pyrococcus furiosus DNA polymerase I will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3620161