Purified thermostable Pyrococcus furiosus DNA ligase

Details Purified thermostable Pyrococcus furiosus DNA ligase Purified thermostable Pyrococcus furiosus DNA ligase

1993-07-22

1996-04-09

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 712, 4351723, 435183, 43525233, 4353201, 435822, 536 231, 536 232, 935 14, 935 29, 935 56, 935 72, 935 73, 935 77, 935 78, C12N 1552, C12N 900, C12N 121, C12N 1563

Patent

active

055061370

ABSTRACT:
Purified thermostable DNA ligase is described that catalyzes template-dependent ligation at temperatures of about 30.degree. C. to about 80.degree. C., and which substantially retains its catalytic ability when subjected to temperatures of from about 85.degree. C. to about 100.degree. C. The thermostable DNA ligase has an estimated molecular weight of 50,000 to 70,000 daltons. A preferred thermostable DNA ligase is described that was isolated from the archaebacteria Pyrococcus furiosus. Also described are plasmid vectors for producing recombinant thermostable DNA ligase.

REFERENCES:
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