Purified thermostable nucleic acid polymerase enzyme from Thermo

Details Purified thermostable nucleic acid polymerase enzyme from Thermo Purified thermostable nucleic acid polymerase enzyme from Thermo

1995-06-07

1997-04-29

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C12N 912

Patent

active

056248334

ABSTRACT:
A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
Simpson et al., 1990, "Purification and Some Properties of a Thermostable DNA Polymerase From a Thermotoga Species" Biochem. Cell Biol. 68:1292-1296.
Simpson et al., 1990, "Purification of a Thermostable DNA Polymerase From a Thermotoga Species" Ann. New York Acad. Sci. 613:426-428.
Lawyer et al., 1989, "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene From Thermus Aquaticus" J. Biol. Chem. 264(11):6427-6437.
Matsuzawa et al., 1988, "Purification and Characterization of Aqualysin I (a thermophilic alkaline serine protease) Produced by Thermus Aquaticus YT-1" Eur. J. Biochem. 171:441-447.
Ruttiman et al., 1985, "DNA Polymerases From the Extremely Thermophilic Bacterium Thermus Thermophilus HB-8" Eur. J. Biochem 149:41-46.
Gelfand et al., 1989, "Taq DNA Polymerase" PCR Technology, Principles and Applications for DNA Amplification, Chapter 2, pp. 17-22 (1989), Ed. by H. A. Erlich.
Leavitt and Ito, 1989, "T5 DNA Polymerase: Structural-Functional Relationships to Other DNA Polymerases" Proc. Natl. Acad. Sci. USA 86:4465-4469.
Bernad et al., 1989, "A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases" Cell 59:219-228.
Huber et al., 1986, "Thermotoga Maritima sp. nov. Represents a New Genus of Unique Extremely Thermophilic Eubacteria Growing Up to 90" Chem. Abstracts 105:386 (Abstract No. 38901u).
Chien et al., 1976, "Deoxyribonucleic Acid Polymerase From the Extreme Thermophile Thermus Aquaticus" J. Bacteriology 127(3):1550-1557.
Suggs et al., 1981, "Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human B2-Microglobulin" Proc. Natl. Acad. Sci. USA 78(11):6613-6617.
Young and Davis, 1983, "Efficient Isolation of Genes by Using Antibody Probes" Proc. Natl. Acad. Sci. USA 80:1194-1198.
Kaledin et al., 1980, "Isolation and Properties of DNA Polymerase From Extremely Thermophilic Bacterium Thermus Aquatics YT1" Biokhimiya 45(4):644-651.
Barany et al., 1991, "The Ligase Chain Reaction in a PCR World" PCR Methods and Applications 1(1):5-16.
Simpson et al., 1990, "Purification and Some Properties of a Thermostable DNA Polymerase From a Thermotoga Species" Biochem. Cell Biol. 68:1292-1296.
Simpson et al., 1990, "Purification of a Thermostable DNA Polymerase From a Thermotoga Species" Ann. New York Acad. Sci. 613:426-428.

Affiliated with

Also associated with

Rating

Purified thermostable nucleic acid polymerase enzyme from Thermo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified thermostable nucleic acid polymerase enzyme from Thermo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified thermostable nucleic acid polymerase enzyme from Thermo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-705074