Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1993-05-14
1996-02-13
Walsh, Steven G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4352523, 4353201, 930240, 536 232, 536 237, C12N 912, C12N 1554, C12N 1574, C12N 119
Patent
active
054910862
ABSTRACT:
Recombinant DNA sequences encoding the DNA polymerase activity of Pyrodictium species can be used to construct recombinant vectors and transformed host cells for production of the activity. Pyrodictium enzymes for catalyzing 3'.fwdarw.5' exonuclease activity, i.e., proofreading enzymes, are also provided. The Pyrodictium enzymes are useful in DNA amplification procedures and are not irreversibly inactivated by exposure to 100.degree. C. in a polymerase chain reaction.
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Gelfand David H.
Wang Alice M.
Gould George M.
Hoffmann-La Roche Inc.
Petry Douglas A.
Sias Stacey R.
Walsh Steven G.
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