Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1992-11-05
1994-10-04
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4351723, 435183, 935 14, C12N 912, C12N 1500, C12N 900
Patent
active
053526007
ABSTRACT:
A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer containing non-ionic detergents that lends stability to the enzyme.
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Gelfand David H.
Stoffel Susanne
Fleisher Mindy B.
Gould George M.
Hoffmann-La Roche Inc.
Sias Stacey R.
Tramaloni Dennis P.
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