Purified thermostable DNA polymerase obtainable from thermococcu

Details Purified thermostable DNA polymerase obtainable from thermococcu Purified thermostable DNA polymerase obtainable from thermococcu

1991-04-17

1994-06-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 691, 435193, 4352523, 43525233, 4353201, 536 232, C12N 1554, C12N 1567, C12N 1570, C12N 1574

Patent

active

053227859

ABSTRACT:
There is provided an extremely thermostable enzyme obtainable from Thermococcus litoralis. The thermostable enzyme has a molecular weight of about 90,000-95,000 daltons, a half-life of about 60 minutes at 100.degree. C. in the absence of stabilizer, and a half-life of about 95 minutes at 100.degree. C. in the presence of stabilizer, such as octoxynol (TRITON X-100) or bovine serum albumin. The thermostable enzyme possesses a 3'-5' proofreading exonuclease activity. The thermostable enzyme may be native or recombinant and may be used for second-strand cDNA synthesis in cDNA cloning, DNA sequencing, and DNA amplification.

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