Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-01-18
1997-02-11
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435194, 536 232, C12N 912, C12N 1554, C12P 1934
Patent
active
056020112
ABSTRACT:
A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95.degree. C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.
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Olga B. d'Hennezel, "Characterization of a Novel Thermostable DNA Polymerase from a Hydrothermal Submarine Vent Microorganism," North Carolina State University catalogued Apr. 6, 1994.
d'Hennezel Olga B.
Duffaud Guy D.
Jolly James F.
Kelly Robert M.
Luhm Robert A.
Hendricks Keith D.
North Carolina State University
Pharmacia Biotech Inc.
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