Purified retroviral constitutive transport enhancer and its use

Details Purified retroviral constitutive transport enhancer and its use Purified retroviral constitutive transport enhancer and its use

1994-05-20

1996-12-17

Budens, Robert D.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Inactivation or attenuation; producing viral subunits

536 241, 4242071, 4242081, 4351723, C12N 704, C12N 1500, C07H 2104, A61K 3921

Patent

active

055852637

ABSTRACT:
A novel retroviral nucleotide sequence comprising a constitutive transport enhancer which functions to transport mRNA transcripts from the nucleus to the cytoplasm of a cell, wherein the mRNA transcript is either differentially spliced, alternatively spliced, incompletely spliced, or unspliced. Also disclosed is a recombinant attenuated HIV containing only structural proteins gag, pol, and env, wherein the expression of the structural proteins is rev-independent and facilitated by the constitutive transport enhancer.

REFERENCES:
Bray et al., "A Small Element from the Mason-Pfizer Monkey Virus Genome Makes Human Immunodeficiency Virus Type 1 Expression and Replication Rev-Independent", Proc. Natl. Acad. Sci. USA, vol. 91, Feb. 1994, pp. 1256-1260.
Osborne and Silver, 1993, "Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae", Annu. Rev. Biochem. 62:219-254.
Kadowaki et al., 1992, "A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm", Proc, Natl. Acad. Sci. USA 89:2312-2316.
Del Pozzo et al., 1994, "Control of nucleo-cytoplasmic HLA-DRA mRNA partitioning by interaction of a retention signal with compartmentalized proteins", J. Mol. Biol. 240:193-204.
Alonso-Caplen et al., 1992, "Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA", Genes Devel. 6:255-267.
Latchman, D., 1990, "Cell-type-specific splicing factors and the regulation of alternative RNA splicing", New Biol. 2:297-303.
Gomeza et al., 1994, "Cellular distribution and regulation by cAMP of the GABA transporter (GAT-1) mRNA", Molec. Brain Res. 21:150-156.

Affiliated with

Also associated with

Rating

Purified retroviral constitutive transport enhancer and its use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified retroviral constitutive transport enhancer and its use , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified retroviral constitutive transport enhancer and its use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1990560