Drug – bio-affecting and body treating compositions – Lymphokine – Interleukin
Patent
1992-05-06
1995-05-30
Draper, Garnette D.
Drug, bio-affecting and body treating compositions
Lymphokine
Interleukin
530351, 435 6952, C07K 1455, A61K 3820
Patent
active
054198992
ABSTRACT:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction, mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
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Hanisch Wolf
Koths Kirston
Kunitani Michael
Thomson James
Wilson Kenneth
Draper Garnette D.
Fitzgerald David
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