Purified proteins, recombinant DNA sequences and processes for p

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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800295, 800278, 536 236, 536 241, 435468, 435419, 4353201, C12N 1529, C12N 1582, A01H 400, A01H 500

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active

060751843

ABSTRACT:
Purified proteins, DNA sequences that code on expression therefore and recombinant DNA molecules, including hosts transformed therewith, for transforming coffee plants to suppress the expression of caffeine. The DNA sequences and recombinant DNA molecules are characterized in that they code on expression for an enzyme in the pathway for caffeine synthesis in coffee. Coffee plants transformed with DNA molecules that code on transcription for mRNA that is antisense to mRNA that codes on expression for at least one enzyme in the pathway for caffeine biosynthesis.

REFERENCES:
"Are Xanthosine and 7-Methylxanthosine Caffeine Precursors?" by Schulthess & Baumann (Received in revised form Feb. 7, 1995).
"A Visible Marker for Antisense mRNA expression in plants: Inhibition of chlorophyll synthesis with a glutamate-1-semialdehyde aminotransferase antisense gene" by Hofgen, et al. (Proc. Natl. Acad. Sci. USA, vol. 91, pp. 1726-1730, Mar. 1994, Plant Biology).
"Purine and Purine Alkalois Metabolism in Camellia and Coffea Plants" by Suzuki, et al. (Phytochemistry Review Article No. 68. 1992, vol. 31, No. 8, pp. 2575-2584).

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