Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-08-12
1999-02-23
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
536 236, 435232, 435183, 435468, 4353201, 800278, 800283, 800285, 800286, 800298, C12N 902, C12N 988, C12N 1529, C12N 1500, A01H 500, A01H 510
Patent
active
058742697
ABSTRACT:
The invention provides purified proteins, DNA sequences that code on expression therefore and recombinant DNA molecules, including hosts transformed therewith for transforming coffee plants to suppress the expression of enzymes necessary for ethylene synthesis. The DNA sequences and recombinant DNA molecules are characerized in that they code on expression for the enzymes ACC synthase or ACC oxidase that are elements of the pathway for ethylene biosynthesis in coffee plants. Coffee plants are transformed with vectors containing ACC synthase and/or with ACC oxidase DNA sequences that code on expression for the respective mRNA that is antisense to the mRNA for ACC synthase and/or ACC oxidase. The resulting antisense mRNA binds to the respective ACC synthase and/or ACC oxidase mRNA, thereby inactivating the mRNA encoding one or more enzymes in the pathway for ethylene synthesis. The described DNA sequences can also be used to block synthesis of ACC synthase or ACC oxidase using co-suppression. The result in either event is that the transformed plants are incapable of synthesizing ethylne, though other aspects of their metabolism is not affected.
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Moisyadi Istefo
Neupane Kabi Raj
Stiles John I.
Mosher Mary E.
University of Hawai'i
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