Purified protein G from streptococcal bacteria

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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435 681, 435 711, 435272, 435820, 530820, 530825, C07K 1504, C07K 312, C07K 1500, C12P 2100

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active

049488740

ABSTRACT:
A process is disclosed for recovering a cell wall protein, particularly a Fc-receptor, from streptococcal bacteria. In the process, streptococcal bacteria are treated with at least one proteolytic enzyme to solubilize the cell wall protein. A process is also disclosed for recovering Fc-receptor type III (protein G) having selective IgG-binding capability (i.e., no albumin-binding capability) from streptococcal bacteria by pretreating these bacteria with enzymes prior to said enzymatic solubilization.

REFERENCES:
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patent: 4481294 (1984-11-01), Downs
Swanson et al., J. Exp. Med., vol. 130, 1969, pp. 1063-1091.
Lancefield, R. C., J. Immunol., vol. 89, 1962, pp. 307-313.
Hill, R. L., Advan. Protein Chem., vol. 20, 1965, pp. 37-107.
Myhre et al., Infection and Immunity, Sep. 1977, pp. 475-482.
Kronvall, G. The Journal of Immunology, vol. 111, No. 5, 1973, pp. 1401-1406.
Christensen et al., Acta. Path. Mircobiol. Scand. Sect. vol. 84, 1976, pp. 196-202.
Bjorck et al., "Purification and Some Properties of Streptococcal Protein G, a Novel IgG Binding Reagent", J. Immunol, 133 (2) (1984), pp. 969-974.

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