Purified plasmodium and vaccine compositions

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Parasitic organism or component thereof or substance...

Reexamination Certificate

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C424S269100

Reexamination Certificate

active

08043625

ABSTRACT:
Disclosed are substantially purifiedPlasmodiumsporozoites and preparations ofPlasmodiumsporozoites substantially separated from attendant non-sporozoite material, where the preparations ofPlasmodiumsporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purifiedPlasmodiumsporozoites are likewise provided. Methods of purifying preparations ofPlasmodiumsporozoites are also provided.

REFERENCES:
patent: 2005/0220822 (2005-10-01), Hoffman et al.
patent: 2007/0169209 (2007-07-01), Hoffman et al.
The Notification of Transmittal of the International Search Report and Written Opinion of the International Searching Authority issued in Int'l Patent Application No. PCT/US2010/20564, prepared Feb. 17, 2010, and mailed Mar. 9, 2010, 12 pages.
Belnoue, E., et al., “Protective T Cell Immunity Against Malaria Liver Stage After Vaccination with Live Sporozoites Under Chloroquine Treatment,”The Journal of Immunology172:2487-2495 (2004).
Engelmann, S., et al., “TransgenicPlasmodium bergheisporozoites Expressing β-galactosidase for Quantification of Sporozoite Transmission,”Mol Biochem Parasitol. 146(1): 30-37 (Mar. 2006).
Wood, D.E., et al., “The Use of Membrane Screen Filters in the Isolation ofPlasmodium bergheiSporozoites from Mosquitos,”Bulletin of the World Health Organization57(Suppl. 1):69-74 (1979).

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