Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-09-13
1997-09-09
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435195, 4353201, 435325, 435419, 4352523, 536 232, C12N 914, C12N 1555
Patent
active
056655517
ABSTRACT:
Purified DNA sequences that encode a thermostable pyrophosphatase are provided. The purified DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are methods for producing thermostable pyrophosphatase.
REFERENCES:
patent: 5498523 (1996-03-01), Tabor et al.
Hohne et al., 1988, "Kinetic Characterization of a Thermostable Inorganic Pyrophosphatase from Thermus thermophilus" Biomed. Biochim. Acta. 47(12):941-947.
Kolakowski et al., 1988, "Cloning, Molecular Characterization and Chromosome Localization of the Inorganic Pyrophosphatase (PPA) Gene From S. Cerevisiae" Nucleic Acids Research 16(22):10441-10452.
Lahti et al., Dec., 1988, "Cloning and Characterization of the Gene Encoding Inorganic Pyrophosphatase of Escherichia coli K-12" J. Bacteriology 170(12):5901-5907.
Tabor and Richardson, 1990, "DNA Sequence Analysis With a Modified Bacteriophage T7 DNA Polymerase" J. Biological Chemistry 265(14):8322-8328.
Richter and Schafer, 1992, "Purification and Enzymic Characterization of the Cytoplasmic Pyrophosphatase from the Thermoacidophilic Archaebacterium Thermoplasma Acidophilum" Eur. J. Biochem. 209:343-349.
Richter and Schafer, 1992, "Cloning and Sequencing of the Gene for the Cytoplasmic Inorganic Pyrophosphatase from the Thermoacidophilic Archaebacterium Thermoplasma Acidophilum" Eur. J. Biochem. 209:351-355.
Yang and Wensel, 1992, "Molecular Cloning and Functional Expression of cDNA Encoding a Mammalian Inorganic Pyrophosphatase" J. Biological Chemistry 267(34):24641-24647.
Obmolova et al., 1993, "Purification, Crystallization and Preliminary X-ray Analysis of Inorganic Pyrophosphatase from Thermus Thermophilus" J. Mol. Biol. 232:312-313.
Teplyakov et al., 1994, "Crystal Structure of Inorganic Pyrophosphatase From Thermus Thermophilus" Protein Science 3:1098-1107.
Boyer, P.D., The Enzymes, 3rd ed., New York, Academic Press, 1971, Chapter 20, entitled "Inorganic Pyrophosphatase of Escherichia coli".
Sambrook et al. "Molecular Cloning: A Laboratory Manual", 1989, Cold Spring Harbor Press, pp. 11.3-11.19.
Gelfand David Harrow
Wang Alice Ming
Hendricks Keith D.
Johnston George W.
Petry Douglas A.
Roche Molecular Systems Inc.
Sias Stacey R.
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