Purified nitric oxide synthase from rat brain

Details Purified nitric oxide synthase from rat brain Purified nitric oxide synthase from rat brain

1996-07-05

1999-01-05

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435335, 424570, C12N 906, C12N 506, A61K 3530

Patent

active

058561587

ABSTRACT:
A nitric oxide synthase (NOS) protein is obtained from rat brain and purified to a specific activity at least 6,360-fold that of a crude tissue preparation. The NOS protein has a denatured MW of 105 kD determined by SDS-PAGE, a dimeric MW of 230 kD determined by gel filtration and requires FAD, FMN, Ca.sup.2+ and tetrahydropterin for the production of nitric oxide from L-arginine or an arginine-rich peptide, oligopeptide or protein substrate.

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