Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis
Patent
1983-07-11
1985-04-09
Rosen, Sam
Drug, bio-affecting and body treating compositions
Fermentate of unknown chemical structure
Having a known elemental analysis
260112R, 435 29, 514 8, A61K 3702, C07G 700, C12Q 129
Patent
active
045101310
ABSTRACT:
A process for purifying Mullerian Inhibiting Substance (MIS) from testicular tissue which comprises incubating testicular tissue in a culture media in the presence of a protease inhibitor in order to extract MIS from the tissue, chromatographing the extract on an anionic exchange resin followed by chromatographing the biologically active product obtained therefrom on a cationic exchange resin; the biologically active material from the cationic exchange resin is chromatographed by lectin affinity chromatography on wheat germ lectin and then either chromatographed by lectin affinity chromatography on concanavalin A or by triazinyl dye affinity chromatography on a appropriate triazinyl dye; MIS is obtained in 8,000-fold and 15,000-fold purification over original testicular tissue.
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Budzik Gerald P.
Donahoe Patricia K.
Swann David A.
Rosen Sam
The General Hospital Corporation
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