Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-01-24
1998-05-19
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435219, C12N 952, C12N 950
Patent
active
057534853
ABSTRACT:
A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
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Dwulet Francis E.
Ellis Bernice B.
Gill John F.
Jacobsen Linda B.
Smith Marilyn E.
Amick Marilyn L.
Boehringer Mannheim Corporation
Harris Brent A.
Saucier Sandra E.
Young D. Michael
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