Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1998-03-13
1999-11-23
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435219, C12N 952, C12N 950
Patent
active
059898887
ABSTRACT:
A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
REFERENCES:
patent: 3201325 (1965-08-01), Barton
patent: 3705083 (1972-12-01), Chiulli et al.
patent: 3821364 (1974-06-01), Chiulli et al.
patent: 4256836 (1981-03-01), Nonaka et al.
patent: 4431544 (1984-02-01), Atkinson et al.
patent: 4431546 (1984-02-01), Hughes et al.
patent: 4732758 (1988-03-01), Hurion et al.
patent: 4797213 (1989-01-01), Parisus et al.
patent: 4868121 (1989-09-01), Scharp et al.
patent: 4873359 (1989-10-01), Chmurny et al.
patent: 4946792 (1990-08-01), O'Leary
patent: 5079160 (1992-01-01), Lacy et al.
patent: 5116615 (1992-05-01), Gokcen
patent: 5120656 (1992-06-01), O'Leary et al.
patent: 5173295 (1992-12-01), Wehling
patent: 5177017 (1993-01-01), Lin et al.
patent: 5273904 (1993-12-01), Langley
patent: 5322790 (1994-06-01), Scharp et al.
patent: 5332503 (1994-07-01), Lee et al.
patent: 5422261 (1995-06-01), Lee et al.
patent: 5753485 (1998-05-01), Dwulet et al.
"Purification and Partial Characterization of Collagenolytic Enzymes from Clostridium histolyticum," Kono, Biochemistry, vol. 7, No. 3, Mar. 1968 (pp. 1106-1114).
"Consecutive Use of .omega.-Aminoalkylagaroses. Resolution and Purification of Clostripain and Collagenase From Clostridium histolyticum," Kuala, et al., Biochemical and Biophysical Research Communications, vol. 69, No. 2, 1976 (pp. 389-396).
"Five Sepharose-Bound Ligands For The Chromatographic Purification of Clostridium Collagenase and Clostripain," Emod et al., FEBS Letters, vol. 77, No. 1, May 1977 (pp. 51-56).
"The Role of Proteolytic Enzymes Derived From Crude Bacterial Collagenase in the Liberation of Hepatocytes From Rat Liver," Hatton, et al., Eur. J. Biochem, 137, 1983 (pp. 311-318).
Enzymatic Isolation of Cells from Neonatal Calvaria Using Two Purfied Enzymes from Clostridum histolyticum, Hefley, et al., Exp. Cell Res., 149 (1983), pp. 227-236.
"Utilization of FPLC-Purified Bacterial Collagenase for the Isolation of Cells from Bone,"Journal of Bone and Medical Research, vol. 2, No. 6, 1987 (pp. 505-516).
"Protease Activity in Pancreatic Islet Isolation by Enzymatic Digestion," McShane, et al., Diabetes, vol. 38, Suppl. Jan. 1, 1989 pp. 126-128.
"An Analysis of the Role of Collagenase and Protease in the Enzymatic Dissociation of the Rat Pancreas for Islet Isolation," Wolters, et al., Diabetologia 35, 1992, pp. 735-742.
"Purification and Separation of Individual Collagenase of Clostridium histolyticum Using Red Dye Ligand Chromatography," Bond, et al., Biochemistry, vol. 23, No. 13, 1984, pp. 3077-3085.
"Characterization of the Individual Collagenases from Clostridium histolyticum," Bond, et al., vol. 23, 1984, pp. 3085-3091.
"Worthington Enzyme Manual: Enzymes and Related Biochemicals," Worthington Biochemical Corporation, 1988, pp. 93-101.
"Purification and Characterization of Myosin from Bovine Thyroid," Kobayashi, et al., The Journal of Biological Chemistry, vol. 252, No.22 Nov. 25, 1977, pp. 8285-8291.
".gamma.-Glutamyl Hydrolase (Conjaugase)," Silink, et al., The Journal of Biological Chemistry, vol. 250, No. 15, 1975, pp. 5982-5994.
"Protein Purification Using Immobilized Triazine Dyes," Dean, et al., Journal of Chromatography, 165 (1979) 301-319.
"Enzymatic Harvesting of Adult Human Saphenous Vein Endothelial Cells: Use of a Chemically Defined Combination of Two Purified Enzymes to Attain Viable Cell Yields Equal to Those Attained by Crude Bacterial Collagenase Preparations," Suggs, et al., Journal of Vasular Surgery, vol. 15, No. 1, Jan. 1992, pp. 205-213.
"Preparation of Single Smooth Muscle Cells from Guinea Pig Taenia Coli by Combinations of Purified Collagenase and Papin," Maruyama, et al., Journal of Pharmacological Methods, 18, 151-161 (1987).
"Enzyme lsolation and Purification," Patel, Biotechnology Applications and Research, Ed. Cheremisinoff and Ouellette, 1985, Chp. 41, pp. 534-562.
"Enzymatic Isolation of Cells from Bone:Cytotoxic Enzymes of Bacterial Collagenase," Hefley, et al., Am. J. Physiol, 240, C234-C238, 1981.
Dwulet Francis E.
Ellis Bernice B.
Gill John F.
Jacobsen Linda B.
Smith Marilyn E.
Harris Brent A.
Roche Diagnostics Corporation
Saucier Sandra E.
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