Purified insulin mediators and purification process for same

Details Purified insulin mediators and purification process for same Purified insulin mediators and purification process for same

1989-03-08

1992-06-16

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536119, 514103, 514866, 514573, 514 23, 530395, 530397, 435232, 436161, 436162, C07K 1500, C07G 700, C12N 988, A01N 5700

Patent

active

051226030

ABSTRACT:
A method for purifying two distinct insulin mediators to substantial homogeneity, and relative purity values above 80%, comprises adsorption on first anion exchange resin and subsequently on a chelex cation exchange resin column. The chelex resin ion exchange column substantially increases the activity of the recovered mediator. Following purification, the already treated fraction is subjected to three successive thin layer chromatography purification steps, the last giving, in the presence of ninhydrin stain, a characteristic salmon-color spot, which is indicative of the presence of the mediator. This process can also be used as a screening process, the absence of the salmon-colored spot being indicative of the diabetic state. Structure information on the insulin mediators obtained is given.

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Thakkar, et al.; Journal Biol Chem; (4-1990); 265(10) pp. 5475-5481.
Niwa, et al; Journal Chromatography; (10-83); 277 pp. 25-39.
Mato, et al; Journal of Biological Chemistry; (262) pp. 2131-2137 (1987).

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