Purified inducible nitric oxide synthase flavoprotein

Details Purified inducible nitric oxide synthase flavoprotein Purified inducible nitric oxide synthase flavoprotein

1991-09-26

1992-07-21

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Glycoprotein, e.g., mucins, proteoglycans, etc.

435195, 435212, 435226, 435228, 530397, C07K 1506, C12N 900, C12N 948, C12N 964

Patent

active

051324075

ABSTRACT:
Inducible nitric oxide (NO) synthase flavoprotein purified to an activity more than 400-fold from activated mouse macrophage cell line is water soluble, has a denatured molecular mass as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions ranging from 125 to 135 kDa, has a molecular mass in catalytically active form of about 250 kDa, does not require added calcium ions or calmodulin for activity, and on heat denaturation releases flavin adenine dinucleotide in an amount of 1.1.+-.0.1 mol per mol of 130 kDa polypeptide subunit of the purified flavoprotein and flavin mononucleotide in an amount of at least 0.55.+-.0.04 mol per mol of 130 kDa polypeptide subunit of the purified flavoprotein.

REFERENCES:
Sakuma et al, Proc. Natl. Acad. Sci. USA 85:8664-8667, 1988.
Iyengar, R., et al Proc. Natl. Acad. Sci, USA, vol. 84, 6369-6373 (Sep. 1987).
Gross, S. S., et al, Biochem. Biophys. Res. Comm., 170, No. 1, 96-103 (Jul. 1990).
Kwon, N. S., et al, J. Biol. Chem. 264, No. 34, 20496-20501 (Dec. 1989).
Kwon, N. S., et al, J. Biol. Chem. 265, No. 23, 13442-13445 (Aug. 1990).
Moncada, S., et al, Biochem Pharmacol, 38, No. 11, 1709-1715 (1989).
Nathan, Carl F., et al, J. Natl. Cancer Inst., 82, No. 9, editorial (May 1990).
Stuehr, D. J. et al, Proc. Natl. Acad. Sci. USA, 82, pp. 7738-7742 (Nov. 1985).
Stuehr, D. J., et al, The Journal of Immunology, 139, No. 2,518-525 (Jul. 1987).
Stuehr, D. J., et al, J. Exp. Med., 169, 1011-1020 (Mar. 1989).
Stuehr, D. J., et al, J. Exp. Med., 169, 1543-1555 (May 1989).
Stuehr, D. J., et al, Biochem. Biophys. Res. Comm., 161, No. 2, 420-426 (Jun. 1989).
Stuehr, D. J., et al, Endothelium-Derived Relaxing Factors (Rubanyi, G. M., et al, eds.), 75-84, S. Karger, Basel, Switzerland, 1990.
Stuehr, D. J., et al, Biochem. Biophys. Res. Comm., 168, No. 2, 558-565 (Apr. 1990).
Stuehr, D. J., et al, The FASEB Journal, vol. 5, 98-103 (Jan. 1991).
Stuehr, D. J., et al, J. Biol. Chem, 266, No. 10, 6259-6263 (Apr. 1991).
Stuehr, D. J., et al, Proc. Natl. Acad. Sci. U.S.A., 88, 7773-7777 (Sep. 1991).
Billiar, T. R., et al, J. Exp. Med., 169, 1467-1472 (Apr. 1989).
Billiar, T. R., et al, Surgery, 106, No. 2, 364-372 (Aug. 1989).
Bredt, D.S., et al, Proc. Natl. Acad. Sci. USA, 87, 682-685 (Jan. 1990).
Curran, R.D., et al, J. Exp. Med., 170, 1769-1774 (Nov. 1989).
Ding, A. H., et al, The Journal of Immunology, 141, No. 7, 2407-2412 (Oct. 1988).
Ding, A. H., et al, The Journal of Immunology, 145, No. 3, 940-944 (Aug. 1990).
Yui, Y., et al, The Journal of Biological Chemistry, vol. 266, No. 19, 12544-12547 (Jul. 5, 1991).
Drapier, J. C., et al, J. Immunol. 140, 2829-2838 (1988).
Mayer B., et al, FEBS Lett., 277, 215-219 (1991).
Schmidt, H. H. H. W., et al, Proc. Natl. Acad. Sci. USA 88, 365-369 (1991).
Tayeh, M. A., et al, J. Biol. Chem. 264, 19654-19658 (1989).
Yui, Y., et al, J. Biol. Chem. 266, 3369-3371 (1991).

Affiliated with

Also associated with

Rating

Purified inducible nitric oxide synthase flavoprotein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified inducible nitric oxide synthase flavoprotein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified inducible nitric oxide synthase flavoprotein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-844361