Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2007-07-17
2007-07-17
Ramirez, Delia M. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S019000, C435S069100, C435S320100, C435S325000, C435S252300, C530S350000, C536S023200
Reexamination Certificate
active
10796669
ABSTRACT:
Disclosed is a novel type II restriction endonuclease. Such enzyme recognizes a particular non-palindromic sequence of 5 oligonucleotides and cleaves DNA downstream of the DNA recognition sequence of nucleotides at the fourth base in the upper strand and the fifth base in the lower strand, and forms a one-base protruding end in the 5′-end after cleavage. The recognition and cleavage site of HpyC1I is identical to the known restriction endonuclease BccI respectively, but the nucleotide sequence and the amino acid sequence are different from any other know restriction enzymes.
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Lin Tzu-Lung
Wang Jin-Town
National Taiwan University
Ostrolenk Faber Gerb & Soffen, LLP
Ramirez Delia M.
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