Purified herpes simplex virus protease and methods of purificati

Details Purified herpes simplex virus protease and methods of purificati Purified herpes simplex virus protease and methods of purificati

1994-07-21

1996-01-23

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352351, 435815, C12N 950

Patent

active

054864704

ABSTRACT:
A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic cleavage occurs, resulting in mature protease.

REFERENCES:
EM Seperations Technology, published by E. Merck, Darmstadt, Germany, 1994, pp. 63-67.
Welch et al., 1993 "Herpes Proteinase: Site-Directed Mutagenesis . . . " J. Virology 67(12): 7360-7372.
Weinheimer et al. 1993 "Autoproteolysis of Herpes Simplex Virus Type I Protesase . . . " J. Virology 67(10): 5813-5822.
DiIanni et al. 1993 "In Vitro Activity of the Herpes Simplex Type I Protease . . . " J. Biol. Chem. 268(34): 25449-25454.

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