Purified forms of DNase

Details Purified forms of DNase Purified forms of DNase

2005-08-23

2005-08-23

Prouty, Rebecca E. (Department: 1652)

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

C435S199000

Reexamination Certificate

active

06932965

ABSTRACT:
The present invention provides the identification and characterization of two components of a recombinant preparation of DNase. These components are the purified deamidated and non-deamidated human DNases. Taught herein are the separation of these components and the use of the non-deamidated species as a pharmaceutical per se, and in particular in compositions wherein the species is disclosed within a plastic vial, for use in administering to patients suffering from pulmonary distress.

REFERENCES:
patent: 2801956 (1957-08-01), Baumgarten et al.
patent: 2834710 (1958-05-01), Baumgarten et al.
patent: 3208908 (1965-09-01), Maxwell et al.
patent: 3663690 (1972-05-01), Eichel et al.
patent: 4065355 (1977-12-01), Khouw et al.
patent: 5077211 (1991-12-01), Yarosh
patent: 5279823 (1994-01-01), Frenz et al.
patent: WO 90/07572 (1990-12-01), None
Ayvazian et al., “The Use of Parenterally Administered Pancreatic Desoxyribonuclease as an Adjunct in the Treatment of Pulmonary Absceases”Am. Rev. of Tuberculosis and Pulmonary Diseases76(1) :1-21 (Jul. 1957).
Borchert et al., “Accelerated Extractable Studies of Borosilicate Glass Containers”Journal of Parenteral Science&Technology43 (2) :67-79 (Mar.-Apr. 1989).
Bradbury et al., “Peptide amidation”TIBS16:112-115 (1991).
Cliffton et al., “Pancreatic Deoxyribonuclease (Dornase) Aerosol in Treatment of Bronchopulmonary Complications and Tracheitis Sicca”Cancer14(2) :414-420 (Mar.-Apr. 1961).
Contreras et al., “Effect of Plastic Containers on Liquid Preservation of Human Granulocytes. I. With and Without Deoxyribonuclease and Hydrocortisone”Transfusion20(5):519-530 (Sep. Oct. 1980).
Doctor, V.M., “Studies on the Purification and Properties of Human Plasma Deoxyribonuclease I”Archives of Biochem. and Biophysics103:286-290 (1963).
Eipper et al., “Peptide α-Amidation”Ann. Rev. Physiol. 50:333-344 (1988).
Farber et al., “Enzymatic Debridement”J. of Thoracic Surgerypp. 45-54 (1953).
Funakoshi et al., “Clinical Studies on Human Pancreatic Deoxyribonuclease I”Japanese Society of Gastroenterology14(1):48-54 (Feb. 1979).
Funakoshi et al., “Purification and Properties of Human Pancreatic Deoxyribonuclease I”J. Biochem. 82(6):1771-1777 (1977).
Hubbard et al., “A Preliminary Study of Aerosolized Recombinant Human Deoxyribonuclease I in the Treatment of Cystic Fibrosis”New England J. of Medicine326(12):812-815 (Mar. 19, 1993).
Ito et al., “Human Urine DNase I: Immunological Identity with Human Pancreatic DNase I, and Enzymic and Proteochemical Properties of the Enzyme”J. Biochem. 95(5):1399-1406 (1984).
Johnson et al., “The Intravenous Injection of Bovine Crystalline Pancreatic Desoxyribonuclease into Patients”J. Clin. Invest. 33:1670-1686 (1954).
Kossiakoff, A.A., “Tertiary Structure Is a Principal Determinant to Protein Deamidation”Science240:191-194 (1988).
Liao et al., “Bovine Pancreatic Deoxyribonuclease A”Journal of Biological Chemistry248(4) :1489-1495 (1973).
Liao, Ta-Hsiu, “Bovine Pancreatic Deoxyribonuclease D”Journal of Biological Chemistry249:2354-2356 (1974).
Liao, Ta-Hsiu, “Multiple forms of deoxyribonuclease I”Mol. and Cell. Biochem. 34:15-22 (1981).
Lourenco et al., “Clinical Aerosols. II. Therapeutic Aerosols”Arch. Intern. Med. 142:2299-2308 (Dec. 1982).
Love et al., “The Relationship between Human Serum and Human Pancreatic DNase I”J. of Biol. Chem. 254 (24) :12588-12594 (Dec. 25, 1979).
Markey, Francis, “Rapid purification of deoxyribonuclease I using fast protein liquid chromatography”FEBS Letters167(1) :155-159 (1984).
Murai et al., “Purification and Properties of Deoxyribonuclease from Human Urine”Biochimica et Biophysics Acta517:186-194 (1978).
Nefsky et al., “Preparation of immobilized monomeric actin and its use in the isolation of protease-free and ribonuclease-free pancreatic deoxoyribonuclease I”European Journal of Biochemistry179:215-219 (1989).
Paudel et al., “Comparison of the Three Primary Structure of Deoxyribonuclease Isolated from Bovine, Ovine, and Porcine Pancreas”J. of Biol. Chem. 261(34) :16012-16017 (Dec. 5, 1986).
Paudel et al., “Purification, Characterization, and the Complete Amino Acid Sequence of Porcine Pancreatic Deoxyribonuclease”J. of Biol. Chem. 261(34) :16006-16011 (Dec. 5, 1986).
Potter et al., “The Composition of Pulmonary Secretions from Patients With and Without Cystic Fibrosis”Am. J. Dis. Child. 100:493-495 (1960).
Raskin, Philip, “Bronchospasm After Inhalation of Pancreatic Dornase”Am. Rev. of Respiratory Disease98:697-698 (1968).
Rosenstreich et al., “A Human Urine-Derived Interleukin 1 Inhibitor”Jouurnal of Experimental Medicine(Abstract #55-131389 (A) 168:1767-1779 (Nov. 1988).
Salnikow et al., “Bovine Pancreatic Deoxyribonucleases A and C”J. of Biol. Chem. 248(5):1499-1501 (Mar. 10, 1973).
Salomon et al., “Aerosols of Pancreatic Dornase in Bronchopulmonary Disease”Annals of Allergypp. 71-79 (Jan.-Feb. 1954).
Segal et al., “Pancreatic Dornase Aerosols in Brochopulmonary Disease”Annals N.Y. Acad. Sci. 68:138-143 (1957).
Shak et al., “Recombinant Human DNase I Reduces the Viscosity of Cystic Fibrosis Sputum”Proc. Natl. Acad. Sci. USA87(23):9188-9192 (Dec. 1990).
Shields et al., “Cloning of part of the gene for bovine deoxyribonuclease I”Biochemical Society Transactions16:195-196 (1988).
Wang et al., “Preparation of Protease-free and Ribonuclease-free Pancreatic Deoxyribonuclease”J. of Biol. Chem. 253(20) :7216-7219 (Oct. 25, 1978).
Wright, H. Tonie, “Sequence and structure determinants of the nonenzymatic deamidation of asparagine and glutamine residues in proteins”Protein Engineering4(3) :283-294 (1991).
Wroblewski et al., “Presence of Desoxyribonuclease Activity in Human Serum”P.S.E.B.M. 74:443-445 (1950).
Yeager, Jr., Henry, “Tracheobronchial Secretions”Am. J. of Medicine50:493-509 (Apr. 1971).
Cacia, J. et al., “Protein sorting by high-performance liquid chromatography I. Biomimetic interaction chromatography of recombinant human deoxyribonuclease I on polyionic stationary phases”J. Chromatography634:229-239 (1993).
Cipolla et al., “Formulation and aerosol delivery of recombinant DNA derived human DNAse”Abstracts of papers of the 205th American Chemical Society National Meeting205(1) :BIOT143 (1993).
Prenz et al., “Characterization of Human Growth Hormone by Capillary Electrophoresis”J. Chromatography480:379-391 (1989).
Stiyaku, Amano, “Patent Abstracts of Japan (Derwent World Patent Index (WPI) Accession No. 80-85035C”Abstract of JP 55-131389(Abstract only ) 5(4) C 38 (Oct. 13, 1980).
Teshima et al., “Deamidation of Soluble CDr at Asparagine-52 Results in reduced Binding Capacity for the NIV-1 Envelope Glycoprotein gp120”Biochemistry30:3916-3922 (1991).
Cacia, J et al., “Protein sorting by high-performance liquid chromatography I. Biomimetic interaction chromatography of recombinant human deoxyribonuclease I on polyionic stationary phases”J Chromatography634:229-299 (1999).
Prenz, J. et al “Characterization of human growth hormone by capillary electrophoresis”J. Chromatography480:379-391 (1989).
Seiyaku, Amano, Derwent World Patent Index (W?I) Accession No. 80-85025C (Oct. 13, 1980).
Teshima, G. et al., “Deamidation of Soluble CD4 at Asparagine-52 results in reduced binding capacity for the HIV-1 envelope glycoprotein gp120”Biochemistry30:3516-3922 (1991).

Affiliated with

Also associated with

Rating

Purified forms of DNase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified forms of DNase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified forms of DNase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3455040