Purified enzyme and process therefor

Details Purified enzyme and process therefor Purified enzyme and process therefor

1986-09-10

1988-06-28

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435 47, 435 49, 435188, 435189, 435814, 435815, C12N 900, C12N 996, C12N 902, C12P 3500, C12D 3506

Patent

active

047538813

ABSTRACT:
Deacetoxycephalosporin C synthetase is provided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chromatography, all carried out in the presence of glycerol, a C.sub.1 -C.sub.3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithiothreitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.

REFERENCES:
patent: 4178210 (1979-12-01), Demain et al.
patent: 4307192 (1981-12-01), Demain et al.
A. Scheidegger et al., Journal of Antibiotics, 37, 522-531 (1984).
J. Kupka et al., FEMS Microbiology Letters, 16 (1983), 1-6.
S. E. Jensen et al., J. Antibiotics, 38, 263-265 (1985).
S. E. Jensen et al., Antimicrobial Agents Chemother., 24, 307-312 (1983).
C. Lubbe et al., Enzyme and Microb. Technol., 1985, vol. 7, Jul., pp. 353-356.

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