Purified DNA polymerase from Bacillus stearothermophilus

Details Purified DNA polymerase from Bacillus stearothermophilus Purified DNA polymerase from Bacillus stearothermophilus

1998-08-18

2000-05-23

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C12N 912

Patent

active

060664835

ABSTRACT:
Composition and methods for the expression of recombinant DNA polymerase enzymes derived from Bacillus stearothermophilus. The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes, compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5436326 (1995-07-01), Ishino et al.
patent: 5466591 (1995-11-01), Abramson et al.
Joyce, C., and N.D.F. Grindley, Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proc. Natl. Acad. Sci. USA 80:1830-1834 (1983).
Lawyer, F.C., et al. High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. In Research: PCR Methods and Applications 2:275-287, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1993).
Livingston, D.M. et al. Affinity Chromatography of Avian Type C Viral Reverse Transcriptase: Studies with Rous Sarcoma Virus Transformed Rat Cells. Virology 50:388-395 (1972).
Naheuer, H.P. and F. Grosse, Immunoaffinity-Purified DNA Polymerase .varies. Displays Novel Properties Biochemistry 26:84588466 (1987).
Pisani, F.M., et al. A DNA polymerase from the archeon Sulfolobus solfataricus hows sequence similarity to family B DNA polymerases. Nucleic Acids Res. 20(11):2711-2716 (1992).
Spadari, S. and A. Weissbach. HeLa Cell R-Deoxyribonucleic Acid Polymerases. J. Biol. Chem. 249(18):5809-5815 (1974).
Uemori, T., et al. Organization and nucleotide sequence of the DNA polymerase gene from the archeon Pyrococcus furiosus. Nucleic Acids Res. 21(2):259-265 (1993).
Uemori, et al., Cloning of the DNA Polymerase Gene of Bacillus caldotenax and Characterization of the Gene Product. J. Biochem. 113:401-410 (1993).
Joyce, et al. Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli. J. Mol. Biochem. 186:283-293 (1985).
Phang, S., et al., Cloning and complete sequence of the DNA ploymerase-encoding gene (Bstpol I) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus, Gene, 163:65-86 (1995).
Lu, Y., et al., Large Fragment of DNA Polymerase I from Bacillus stearothermophilus (Bst Polymerase) is Stable at Ambient Temperature, Biotechniques, 11(4):464, 466 (1991).
McClary, J., et al., Sequencing with the large fragment of DNA polymerase I from Bacillus stearothermophilus, DNA Sequence, 1:173-180 (1991).
Lawyer, F., et al., Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus, J. of Biological Chemistry, 264(11):6427-6437 (1989).
Bernad, A., et al., A Conserved 3'--5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases, Cell, 59:219-228 (1989).
Biological Abstracts, vol. BA84, Philadelphia, PA, USA; Abstract No.: 97671, Ye, S., et al., Heat-Stable DNA Polymerase I Large Fragment Resolves Hairpin Structure In DNA Sequencing.
Database WPI, Derwent Publications Ltd., London, G.B.; AN 88-203377 & SU-A-1 311251 (Lengd Nucler Phys), Jan. 30, 1988 (Abstract).
Stenesh, et al., Biochem. Biophys. Acta, 272:156-166 (1972).
Kaboev, et al., J. Bact., 145(1):21-26 (1981).
Kaboev et al. J. Bact., 145: 21-26, 1981.
Phang et al. Gene, 163: 65-68, 1995.

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