Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1992-11-30
1994-12-20
Wityshyn, Michael G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530300, 530322, 530371, 530395, 530415, 530417, 530422, 530823, 5361231, 210656, 210658, 210660, C07K 300, B01D 1508
Patent
active
053747143
ABSTRACT:
A method of obtaining a novel polypeptide from a crude extraction product of polysaccharide peptide Coriolus versicolor comprising: a) boiling a water soluble powder of polysaccharide peptide Coriolus versicolor; b) centrifuging a boiled product from step a); c) filtering a centrifuged product from step b); d) purifying a solution from step c) by gel filtration chromatography; e) subjecting the purified material from step d) to HPLC using a reversed-phase at ambient temperature, wherein a solvent composition is at an acidic Ph and further includes KCl solvent, and wherein an elution system consists of a linear gradient of about 80% methanol applied at a rate of from between 0-40 minutes to obtain chromatographic peaks; f) analyzing the chromatographic peaks by monitoring for absorbance at about 230 nm, 1.0 AUFS for protein analysis and about 620 nm, 0.02 AUFS for polysaccharide analysis and collecting fractions of each peak in a microfractionator; g) filter-sterilizing each eluent of each chromatograph fraction peak, and drying product therefrom under reduced pressure; h) preparing a solution of an ampholyte mixture of the dried product and identifying structural components of the product by capillary isoelectrophoresis; and i) using gel filtration to obtain polypeptides products of from about 10K and to 50K.
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Yang et al, Am. J. Chim. Med, vol. 20, No. 3-4, pp. 221-232, 1992 (only abstract provided).
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Cho et al, Chemical Abstract, vol. 110, p. 333, Ref. No. 228285x, 1989.
Chen George
Yang Mable M. P.
Mohamed Abdel A.
Wityshyn Michael G.
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