Purified bacterial protein which stimulates ligation of nucleic

Details Purified bacterial protein which stimulates ligation of nucleic Purified bacterial protein which stimulates ligation of nucleic

1990-05-30

1993-07-20

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 435183, 43525233, 4353201, C12N 1533, C12N 1552, C12N 1570, C12N 900

Patent

active

052292738

ABSTRACT:
The subject invention provides a purified protein having a molecular weight of about 22.8 kDa and the amino acid sequence shown in FIG. 2. The invention further provides a method of producing the 22.8 kDa protein which comprises culturing the host cell comprising an expression vector under conditions such that the expression vector contained therein expresses the protein and recovering from the host cell the protein so expressed. Also provided is a method of ligating separate nucleic acid molecules which comprises contacting the molecules with the 22.8 kDa protein under conditions such that the molecules are ligated together.

REFERENCES:
B. E. Windle et al., Proc. Natl. Acad. Sci. USA 83:3885-3889, Jun. 1986.
B. E. Windle et al., J. of Bacteriology 170:4881-4889, Oct. 1988.
S. D. Lu et al., J. of Bacteriology 171:3427-3442, Jun. 1989.

Affiliated with

Also associated with

Rating

Purified bacterial protein which stimulates ligation of nucleic does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified bacterial protein which stimulates ligation of nucleic , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified bacterial protein which stimulates ligation of nucleic will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1759788