Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1987-12-17
1989-08-22
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
424 855, 424101, 514 8, 514 21, 530395, 530830, C07K 1514, A61K 3516, A61K 3702
Patent
active
048597660
ABSTRACT:
The selective appetite-regulating substances satietin and satietin-D had up to the present been prepared from human or animal blood serum or plasma by ultrafiltration, gel chromatography, treatment with trichloroacetic acid, affinity chromatography and optionally treatment with a proteolytic enzyme.
According to the invention, satietin and satietin-D are obtained in a purified form by using immunoabsorption. Being glycoproteins, both satietin and satietin-D form antibodies (antisatietin and antisatietin-D, respectively) in the living organism and can be isolated from the blood and bound to a gel column. For the preparation of satietin and satietin-D, respectively, the human or animal blood plasma or serum is subjected to ultrafiltration and the ultrafiltrate, containing in addition to satietin and satietin-D other constituents with a molecular weight below 50000 daltons, is contacted with the gel column. The antibody bound to the gel column forms with satietin or satietin-D, respectively, an immunocomplex remaining on the column while the other constituents of the ultrafiltrate can be eluted by a buffer. Then, the immunocomplex is cleaved, whereby satietin or satietin-D, respectively, can be eluted in a purified form from the column. The column containing the antibody may repeatedly be used.
REFERENCES:
patent: 4294825 (1981-10-01), Knoll et al.
patent: 4430264 (1984-02-01), Knoll et al.
patent: 4588685 (1986-05-01), Knoll et al.
Harmath Sandor
Knoll Berta
Knoll Jozsef
Nagy Janos
Dubno Herbert
Myers Jonathan
Richter Gedeon Vegyeszeti
Schain Howard E.
LandOfFree
Purified appetite-regulating substances of biological orgin, the does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purified appetite-regulating substances of biological orgin, the, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified appetite-regulating substances of biological orgin, the will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2418183