Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1998-08-05
1999-11-23
Tsang, Cecilia J.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
514 20, 514 21, 530359, 530361, 530402, 530421, 530427, 435 691, 435 696, 424406, A61K 3800, C07K 100
Patent
active
059900814
ABSTRACT:
A substantially endotoxin-free ApoA or ApoE is produced by recombinant DNA technique, suitably in E. coli. Medicaments and methods for treatment of atherosclerosis or cardiovascular diseases employ ApoA or ApoE purified by a process comprising contacting a first aqueous solution comprising ApoA or ApoE and endotoxins with a matrix comprising an immobilized compound with an end group comprising two or three nitrogen atoms bonded to a carbon atom for attaching the endotoxins to the matrix, and subsequently treating the matrix comprising the immobilized compound with a second aqueous solution comprising a surfactant for releasing the ApoA or ApoE while the endotoxins remain attached to the matrix. Alternatively, the ApoA or ApoE is purified by a process comprising contacting the first aqueous solution comprising ApoA or ApoE and endotoxins with an anion-exchange matrix for attaching the ApoA and ApoE to the matrix, treating the anion-exchange matrix with a second aqueous solution comprising a compound comprising two or three nitrogen atoms bonded to a carbon atom for releasing the endotoxins while the ApoA or ApoE remains attached to the matrix, and then releasing the ApoA or ApoE from the matrix.
REFERENCES:
patent: 4740304 (1988-04-01), Tjerneld et al.
patent: 5059528 (1991-10-01), Bollen et al.
patent: 5089602 (1992-02-01), Isliker et al.
patent: 5128318 (1992-07-01), Levine et al.
patent: 5834596 (1998-11-01), Ageland et al.
F.B. Anspach et al, Removal of Endotoxins by Affinity Sorbents Journal of Chromatography A, 711 (1995) 81-92.
S. Minobe et al, Characteristics and Applications of Adsorbents for Pyrogen Removal Biotechnology and Applied Biochemistry 10, 143-153 (1988).
H.B. Brewer et al, The Amino Acid Sequence of Human APOA-I, an Apolipoprotein Isolated from High Density Lipoproteins Biochemical and Biophysical Research Communications, 80:3 (1978) 623-630.
J.P. Segrest et al, A Molecular Theory of Lipid-Protein Interations in the Plasma Lipoproteins FEBS Letters, 38:3 (1974) 247-253.
G. Franceschini et al, A-IMilano Apoprotein: Decreased High Density Lipoprotein Cholesteral Levels with Significant Lipoprotein Modifications and Without Clinical Atherosclerosis in an Italian Family J Clin. Invest. 66 (1980) 892-900.
K.H. Weisgraber et al, Apolipoprotein A-IMilano: Detection of Normal A-I in Affected Subjects and Evidence for a Cysteine for Arginine Substitut in the Variant A-I J. Biological Chemistry, 258:4 (1983) 2508-2513.
G. Franceschini et al, Apoliporotein A-IMilano: Accelerated Binding and Disassociation from Lipids of a Human Apolipoprotein Variant J. Biological Chemistry, 260:30 (1985) 16321-16325.
K. Emancipator et al, In Vitro Inactivation of Bacterial Endotoxin by Human Lipoproteins and Apolipoproteins Infection and Immunity,, 60:2 (1992) 596-601.
R. Munford et al, Binding of Salmonella Typhimurium Lipopolysaccharides to Rat High-Density Lipoproteins Infection and Immunity, 34:3 (1981) 835-843.
T. Karplus et al, A new Method for Reduction of Endotoxin Contamination from Protein Solutions J. Immuno. Methods, 105 (1987) 211-220.
C.R. Sirtori et al, Human Apolipoprotein Mutants III: Crystal Structures of the Common Apolipoprotein E Variants: Insights into Functional Mechanisms, NATO ASI Series, Springer Verlag, Berlin, vol. II, 73-96 (1993).
Japio Abstract of JP61-96998 (1986).
Abstract of JP1-95798 (1989).
Freudenberg et al (1979) Nat. Toxins, Proc. Inc. Symp. Anim., Plant Microb. Toxins., 6th Edition, pp. 349-354.
Gualandri et al (1985), Am. J. Hum. Genet., vol. 37, pp. 1083-1097.
Ulevitch et al (1981) J. Clin. Invest., vol. 67, pp. 827-837.
Sharma (1986) Biotechnology and Applied Biochemistry, vol. 8, pp. 5-22.
Matsumae et al (1990) Biotechnology and Applied Biochemistry, vol. 12, pp. 129-140.
Deeb et al (1991) J. Biological Chemistry, vol. 266, No. 21, pp. 13654-13660.
Takada et al (1991) Journal of Lipid Research, vol. 32, pp. 1275-1280.
Matsunaga et al (1991) Proc. Natl. Acad. Sci. USA, vol. 88, pp. 2793-2797.
Weisgraber et al (1980) J. Clin. Invest., vol. 66, pp. 901-907.
Alred et al., "Application of temperature-induced phase partitioning at ambient temperature for enzyme purification," J Chromatogr A 659(2):289-98 (1994).
Alred et al., Synthesis of dye conjugates of ethylene oxide-propylene oxide copolymers and application in temperature-induced phase partitioning, Bioseparation 2(6):363-73 (1992).
Alred, et al., "Partitioning of ecdysteroids using temperature-induced phase separation," J. Chromatography 628:205-214 (1993).
Badimon, et al., "Regression of atherosclerotic lesions by high density lipoprotein plasma fraction in the cholesterol-fed rabbit," J. Clin Invest. 85(4):1234-41 (1990).
Berggren, et al., "Effects of salts and the surface hydrophobicity of proteins on partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide-propylene oxide copolymers," J. Chromatography 718: 67-79 (1995).
Bradford, et al., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding," Anal Biochem72:248-54 (1976).
Calebrisi, et al., "Molecular characterization of native and recombinant apolipoprotein A-IMilano dimer. The introduction of an interchain disulfide bridge remarkably alters the physicochemical properties of apolipoprotein A-I," J Biol Chem. 269(51): 32168-74 (1994).
Cohn, et al., "Preparation and Properties of Serum and Plasma Proteins. IV. A System for the Separation into Fractions of the Protein and Lipoprotein Components of Biological Tissues and Fluids," J. Am. Chem. Soc. 68: 459-475 (1946).
Eisenberg, et al., "A helical hydrophobic moment: a measure of the amphiphilicity of a helix," Nature 299: 371-374 (1982).
Fransechini, et al. "Apolipoprotein AIMilano. Disulfide-linked dimers increase high density lipoprotein stability and hinder particle interconversion in carrier plasma," J Biol Chem. 265(21):12224-31 (1990).
Fransechini, et al. "High density lipoprotein-3 heterogeneity in subjects with the apo-AIMilano variant," J. Biol. Chem. 257(17): 9926-30 (1982).
Harris, et al. "Enzyme purification using temperature-induced phase formation," Bioseparation. 2(4): 237-46 (1991).
Index, 11eEd., Merck and Co., Rahway, NJ, pp. 342 and 455 (1989).
Isaachi, et al., "Mature apolipoprotein AI and its precursor proApoAI: influence of the sequence at the 5' end of the gene on the efficiency of expression in Escherichia coli," Gene 81(1):129-37 (1989).
Johansson, et al., "Effects of Salts on the Partition of Proteins in Aqueous Polymeric Biphasic Systems," Acta Chimica Scandivica B 28: 873-882 (1974).
Lerch, et al., "Isolation and Properties of Apolipoprotein A for Therapeutic Use," Protides Biol. Fluids 36: 409-416 (1989).
Medzour, et al. "Anion-exchange fast protein liquid chromatographic characterization and purification of apolipoproteins A-I, A-II, C-I, C-II, C-III0, C-III1, C-III2 and E from human plasma," J Chromatogr. 414(1):35-45 (1987).
Moguilevsky, et al., "Production of human recombinant proapolipoprotein A-I in Escherichia coli: purification and biochemical characterization," DNA 8(6):429-36 (1989).
Nilsson, et al., "Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors," EMBO J. 4(4):1075-80 (1985).
Nitschmann, et al., "Vereinfachtes Verfahen zur Gewinnung von humanem Albumin und--Globulin aus Blutplasma mittels Alkoholfallung," Helv. Chim. Acta. 37:866-873 (1954).
Oncley, et al., "The separation of the Antibodies, Isogglutinins, Prothrombin, Plasminogen and .beta.1--Lipoprotein into Subfractions of Human Plasma," J. Am. Chem. Soc. 71: 541-550 (1949).
Peitsch, et al., "A purification method for apolipoprotein A-I and A-II," Anal Biochem. 178(2):301-5 (1989).
Pigiet, et al., "Thioredoxin-catalyzed refolding of disulfide-containing proteins," Proc Natl Acad Sci U S A. 83(20):7643-7 (1986).
Ross, et al., "Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma," Anal Biochem 149(1):166-8 (1985).
Rubenstein, et al., "A new method for the fractionation of human plasma high density lipoprotein," C
Ageland Hans
Romander Lena
Esperion Therapeutics, Inc.
Mohamed Abdel A.
Tsang Cecilia J.
LandOfFree
Purified APO A and APO E compounds and methods for using them does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purified APO A and APO E compounds and methods for using them, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified APO A and APO E compounds and methods for using them will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1221934