Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1988-08-03
1993-10-12
Lacey, David L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
53038825, 43524027, C07K 1528, C12N 518
Patent
active
052527123
ABSTRACT:
Antibodies which form immune complexes with human native prothrombin only, in the presence of mixtures of human native prothrombin and human abnormal prothrombin as well as antibodies which form antibody antigen complexes with human abnormal prothrombin in the presence of such mixtures have been obtained. Immunoassay techniques are used for qualitative and quantitative determinations of these antigens in human plasma or serum. Unique methods of obtaining the antibodies are described including obtaining antibodies to native prothrombin by dissociation of antigen antibody complexes formed in the presence of calcium ions with a material having a greater affinity constant for binding with calcium ions than does prothrombin. Dissociation of the complex in this manner yields human native prothrombin antibodies which are specific and non-reactive with human abnormal prothrombin. A process is described in which assays are applied to the sensitive detection of vitamin K deficiency and various forms of liver disease including hepatocellular carcinoma, and to monitoring of anticoagulant therapy with sodium warfarin. The invention described herein was made in the course of working under a grant from the Department of Health and Human Services.
REFERENCES:
Lewis et al., "Hybridoma-Produced Conformation-Specific Monoclonal Antibodies to Human Prothrombin," Blood (Suppl. 1), 58:220a (Nov. 1981).
Lewis et al., "Conformation-Specific Monoclonal Antibodies Directed Against the Calcium-Stablized Structure of Human Prothrombin," Biochem; 22:948-954 (1983).
Blanchard et al., "Antibodies Specific for Bovine Abnormal (Des-.gamma.-Carboxy) prothrombin," J. Biol. Chem., 254(24):12513-12520 (1979).
Gantdtt et al., "Plasma Prothrombin during Treatment with Dicumarol," Scand. Jour. Clin & Lab. Invest, 22:23-28 (1968).
Blanchard et al., The New England Journal of Medicine, "Acquired Vitamin K-Dependent Carboxylation Deficiency in Liver Disease," 305: 242-248 (1981).
Liebman et al., "Hepatic Vitamin K-Dependent Carboxylation of Blood-Clotting Proteins," Hepatology 2: 488-494 (1982).
Furie et al., "Radioimmunoassays of Species of Human Prothrombin," in Disroders of Thrombin Formation, Colman, ed., 1983, pp. 84-100.
Blanchard et al., "Immunoassays of Human Prothrombin Species which Correlate with Functional Coagulant Activities", J. of Lab. and Clinical Medicine, 101: 242-255 (1983).
Blanchard et al., "Subclinical Vitamin K Deficiency in Newborns and their Mothers," Blood, 62: 272a (1983), Abstr. No. 994.
Furie et al., "Clinical Applications for Immunoassays of Abnormal and Native Prothrombin," Hematology, Ann. Mtg. Prog., Dec. 3-6, 1983, pp. 57-59.
Liebman et al., "Des-.gamma.-Carboxy (Abnormal) Prothrombin as a Serum Marker of Primary Hepatocellular Carcinoma," The New England J. of Med., 310: 1427-1431 (1984).
Furie et al., "Comparison of the Native Prothrombin Antigen and the Prothrombin Time for Monitoring Oral Anticoagulant Therapy," Blood, 64: 445-451 (1984).
Krasinski et al., "The prevalence of vitamin K definiency in chronic gastrointestinal disorders," The American J. of Clin. Nutrition, 41: 639-643 (1985).
Owens et al., "Monoclonal Antibodies against Human Abnormal (Des-.gamma.-carboxy) prothrombin Specific for the Calcium-free Conformer of Prothrombin," The J. of Bio. Chem., 259: 13800-13805 (1984).
Blanchard et al., "Plasma Prothrombin and Abnormal Prothrombin in Antigen," Blood, 58: 231A (1981), Abstr. No. 833.
Blanchard et al., "Acquired Disorders of Hepatic Vitamin K-Dependent Carboxylation of Prothrombin," Clinical Rearch, 29: 516A (1981).
Furie et al., "Antibodies Directed against a .gamma.-Carboxyglutamic Acid-rich Region of Bovine Prothrombin," The J. of Biol Chem., 253: 8980-8987 (1978).
Tai et al., "Conformation-Specific Antibodies as Probes of the Metal Dependent Tertiary Structure of Bovine Prothrombin," Federation Proceedings, 38: Abstr. No. 2970, (1979).
Yelten, et al., "Plasmacytomas and Hybridomas: Development and Applications," in Monoclonal Antibodies, ed. by Kennett et al. (Plenum Press), p. 19.
Bynum et al., "Low-Dose Heparin Therapy in the Long-Term Management of Venous Thromboembolism," The American J. of Med. 67: 553-556 (1979).
Ganrot, P. O., et al., Scand. Journ. Clin. & Lab. Invest., 21: 238-244, (1968).
Kohler, G., et al., "Continuous cultures of fused cells secreting antibody of predefined specificity," Nature 256: 495-497, (1975).
Furie, B.,
This invention was made with Government support under 5-RO1-HL21543-03 awarded by the National Heart, Lung, and Blood Institute. The Government has certain rights in the invention.
Blanchard Rita A.
Furie Barbara C.
Furie Bruce E.
Futrovsky Susan L.
Lacey David L.
New England Medical Center Hospitals Inc.
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