Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-12-27
1993-10-26
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435221, C12N 956, C12N 954
Patent
active
052565572
ABSTRACT:
A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.
REFERENCES:
patent: 3623957 (1971-11-01), Feldman
patent: 4689297 (1987-08-01), Good et al.
patent: 5041377 (1991-08-01), Becker et al.
Horvat, T. et al. (1987), Eur. Congr. Biotechnol. (2), pp. 605-608.
Henry S. D., Cross Flow Filtration Recent Dvpts., Sep. Science (1972).
Bauer et al., J. Chem., 5(3), pp. 117-120 (1967).
N. C. Beaton, Appl. of Ultrafiltration to Ferment. Prod., Polymer Science Technology, 13 (1980).
Bradford, Anal. Biochem., vol. 72, pp. 248-254 (1976).
Fields, R., Methods In Enzymology, vol. 25, pp. 464-466 (1972).
Priest, F. G., Goodfellow, M. & Todd, C., A Numerical Classication of the Genus Bacillus, Journal of General Microbiology, vol. 134, pp. 1847-1882 (1988).
C. Starace and M. C. Barford, Encyclopedia Chem. Technol., 9, 138-148 (1980).
K. Horikoshi, T. Akika, A New Microbial World, Springer-Verlag, N.Y. p. 93 (1982).
M. Dixon, E. D. Webb, Enzymes, Academic Press, N.Y., pp. 37-41 (1964).
Curline, Methods of Plasma Protein Fractionation, Academic Press, N.Y. (1980).
Nicholson Mary A.
Patel Chimanbhai P.
Shetty Jayarama K.
Robinson Douglas W.
Sevigny Jeffrey J.
Solvay Enzymes, Inc.
LandOfFree
Purified alkaline protease concentrate and method of preparation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purified alkaline protease concentrate and method of preparation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified alkaline protease concentrate and method of preparation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-958705