Purified alkaline protease concentrate and method of preparation

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435221, C12N 956, C12N 954

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active

052565572

ABSTRACT:
A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.

REFERENCES:
patent: 3623957 (1971-11-01), Feldman
patent: 4689297 (1987-08-01), Good et al.
patent: 5041377 (1991-08-01), Becker et al.
Horvat, T. et al. (1987), Eur. Congr. Biotechnol. (2), pp. 605-608.
Henry S. D., Cross Flow Filtration Recent Dvpts., Sep. Science (1972).
Bauer et al., J. Chem., 5(3), pp. 117-120 (1967).
N. C. Beaton, Appl. of Ultrafiltration to Ferment. Prod., Polymer Science Technology, 13 (1980).
Bradford, Anal. Biochem., vol. 72, pp. 248-254 (1976).
Fields, R., Methods In Enzymology, vol. 25, pp. 464-466 (1972).
Priest, F. G., Goodfellow, M. & Todd, C., A Numerical Classication of the Genus Bacillus, Journal of General Microbiology, vol. 134, pp. 1847-1882 (1988).
C. Starace and M. C. Barford, Encyclopedia Chem. Technol., 9, 138-148 (1980).
K. Horikoshi, T. Akika, A New Microbial World, Springer-Verlag, N.Y. p. 93 (1982).
M. Dixon, E. D. Webb, Enzymes, Academic Press, N.Y., pp. 37-41 (1964).
Curline, Methods of Plasma Protein Fractionation, Academic Press, N.Y. (1980).

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