Purification processes for isolating purified vesicular...

Details Purification processes for isolating purified vesicular... Purification processes for isolating purified vesicular...

2011-01-25

2011-01-25

Lucas, Zachariah (Department: 1648)

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Recovery or purification

C424S224100, C424S184100, C424S204100, C424S093600, C424S093100, C435S261000, C435S235100

Reexamination Certificate

active

07875446

ABSTRACT:
A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.

REFERENCES:
patent: 6008036 (1999-12-01), Fanget et al.
patent: 6033886 (2000-03-01), Conzelmann et al.
patent: 6168943 (2001-01-01), Rose
patent: 6261823 (2001-07-01), Tang et al.
patent: 7326555 (2008-02-01), Konz et al.
patent: 2003/0091592 (2003-05-01), Barber
patent: 2003/0175688 (2003-09-01), Pennathur-das et al.
patent: WO 97/06243 (1997-02-01), None
patent: WO 03/078592 (2003-09-01), None
patent: WO 03/093463 (2003-11-01), None
patent: WO 2004/101765 (2004-05-01), None
patent: WO 2005/070953 (2005-08-01), None
patent: WO 2005/080556 (2005-09-01), None
patent: WO 2005/098009 (2005-10-01), None
patent: WO 2006/011580 (2006-02-01), None
Pall Corporation (Online Publication of Dec. 1, 2005), Dynamic High Capacity Mustang® Q Membrane Units for Scaleable Anion Exchange Chromatography Purification of Adenoviral Vectors.
Sartorius Corporation, Membrane Filter Cartridges, Sartobran P 0.2 um, Online Publication, 2003.
Whatman Corporation, Product Guide, 2004-2005.
Frazatti-Gallina et al., Vero-cell rabies vaccine produced using serum-free medium, 2004, Vaccine, vol. 23, pp. 511-517.
Gallione et al., “Nucleotide sequences of the mRNA's encoding the vesicular Stomatitis virus N and NS proteins”, J. Virol., Aug. 1981, 39(2):529-535.
Roberts et al., “Attenuated vesicular Stomatitis virus as vaccine vectors”, J. Virol., May 1999, 73(5):3723-3732.
Robinson et al., “The nucleic acid of the bryan strain of rous sarcoma virus: purification of the virus and isolation of the nucleic acid”, Biochemistry, Jul. 1965, 54:137-144.
Rose et al., “Nucleotide sequences of the mRNA's encoding the vesicular Stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions”, J. Virol., Aug. 1981, 39(2):519-528.
Rose et al., “An effective AIDS vaccine based on live attenuated vesicular Stomatitis virus recombinants”, Cell, Sep. 7, 2001, 106:539-549.
Schlereth et al., “Successful vaccine-induced seroconversion by single-dose immunization in the presence of measles virus-specific maternal antibodies”, J. Virol., May 2000, 74(10):4652-4657.
Zolotukhin et al., “Recombinant adeno-associated virus purification using novel methods improves infectous titer and yield”, Gene Therapy, Jun. 1999, 6:973-985.
Burkhart, Christoph et al., “Characterization of T-Helper Epitopes of the Glycoprotein of Vesicular Stomatitis Virus”, Journal of Virology, vol. 68, No. 3, 1994, pp. 1573-1580.
de las Mercedes Segura, Maria et al., “Downstream processing of oncoretroviral and lentiviral gene therapy vectors”, Biotechnology Advances, vol. 24, 2006, pp. 321-337.
Morenweiser, R., “Downstream processing of viral vectors and vaccines”, Gene Therapy, vol. 12, 2005, pp. S103-S110.
Scherr, M. et al., “Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography”, Gene Therapy, vol. 9, 2002, pp. 1708-1714.
Hecht, T. et al., “Limitation of VSV infection by the host's response to VSV-associated cellular antigens”, The Journal of Immunology, vol. 129, No. 4, 1982, pp. 1736-1741.
Brown et al., “Structure and immunogenicity of antigens derived from the virion by treatment with Tween and ether”, J. Immun., 99(1):171-177, 1967.
Downing et al., “Active respiratory syncytial virus purified by ion-exchange chromatography: characterization of binding and elution requirements”, Journal of Virological Methods, 38(2):215-228, Aug. 1992.
Endres et al., “Evaluation of an ion-exchange membrane for the purification of plasmid DNA”, Biotechnology and Applied Biochemistry, 37(3):259-266, Jun. 2003.
Rabotti & Teutsch et al., “The Immunologic determinants in the system of avian oncogenic virus. Purification and immunochemical characterization of the antigen for the group in chick cells transformed by Rous sarcoma virus (Schmidt-Ruppin strain)”, Comptes Rendus des Seances de l'Academie des Sciences, Serie D: Sciences Naturelles, 272(2):343-346, Jan. 11, 1971.
Haber et al., “Membrane chromatography of DNA: Conformation-induced capacity and selectivity”, Biotechnology and Bioengineering, 88(1):26-34, Oct. 5, 2004.
Jacoli, “Purification of turnip mosaic virus by gel filtration”, Biochimica et Biophysica Acta, General Subjects, 165(2):299-302, Sep. 3, 1968.
KÅrsnäs et al., “Bovine viral diarrhea virus: purification of surface proteins in detergent-containing buffers by fast protein liquid chromatography”, Journal of Chromatography, 266:643-649, Aug. 26, 1983.
Kanani et al., “Protein bioseparation by membrane chromatography using polyelectrolyte gel-coated adsorptive membranes”, AIChE Annual Meeting, Conference Proceedings, Austin, TX, United States, Nov. 7-12, 2004.
Kristiansen et al., “Virus purification by Vicia ervilia lectin coupled to Sepharose”, Protides of the Biological Fluids, 23:663-665, 1976.
Lu et al., “Purification of Hemoglobin by Ion Exchange Chromatography in Flow-Through Mode with PEG as an Escort”, Artificial Cells, Blood Substitutes, and Biotechnology, 32(2):209-227, May 2004.
McSharry & Benzinger, “Concentration and purification of vesicular stomatitis virus by polyethylene glycol precipitation”, Virology, 40(3):745-746, Mar. 1970.
Menard et al., “Bovine leukemia virus: purification and characterization of the aspartic protease”, Virology, 193(2):680-689, Apr. 1993.
Nishimura & Kitaoka, “Purification of Japanese encephalitis virus and its antigenic particles from infected suckling mouse brains”, Japanese Journal of Medical Science & Biology, 17:295-305, Dec. 1964.
Nochumson et al., “Use of ion-exchange membrane adsorbers for production of biopharmaceuticals”, Abstracts of Papers, 221st ACS National Meeting, San Diego, CA, United States, COLL-442, Apr. 1-5, 2001.
Pora & Hall, “Use of ion-exchange membrane adsorbers for production of biopharmaceuticals”, Animal Cell Technology: From Target to Market, Proceedings of the ESACT Meeting, 17th, Tyloesand, Sweden, 466-468, Jun. 10-14, 2001.
Roberts et al., “Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing an Influenza Virus Hemagglutinin Provides Complete Protection from Influenza Virus Challenge”, Journal of Virology, 72(6):4704-4711, Jun. 1998.
Specht et al., “Densonucleosis virus purification by ion exchange membranes”, Biotechnology and Bioengineering, 88(4):465-473, Nov. 20, 2004.
Teeters et al., “Adsorptive membrane chromatography for purification of plasmid DNA”, Journal of Chromatography, A, 989(1):165-173, Mar. 7, 2003.
Transfiguracion et al., “Size-Exclusion Chromatography Purification of High-Titer Vesicular Stomatitis Virus G Glycoprotein-Pseudotyped Retrovectors for Cell and Gene Therapy Applications”, Human Gene Therapy, 14(12):1139-1153, Aug. 10, 2003.
Vellekamp et al., “Empty cap

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