Purification procedure of TPA from crude preparations

Details Purification procedure of TPA from crude preparations Purification procedure of TPA from crude preparations

1990-05-01

1992-08-25

Patterson, Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435815, C12N 964

Patent

active

051418630

ABSTRACT:
Tissue plasminogen activator (tPA) species having a specific molecular weight can be isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with hydroxyapatite and then separately eluting the adsorbed tPA species with eluents having different pHs and/or salt concentrations.

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Ritken, D. et al., J. Biol. Chem., vol. 256, pp. 7035-7041, 1981.
Winkler, M. E., et al., (1986) Biochemistry 25, 4041-4045.

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