Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Reexamination Certificate
2011-08-23
2011-08-23
Mosher, Mary E (Department: 1648)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
C424S232100, C424S199100
Reexamination Certificate
active
08003364
ABSTRACT:
The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.
REFERENCES:
patent: 6761893 (2004-07-01), Chaplin et al.
patent: 2007/0071769 (2007-03-01), Sutter et al.
patent: 2008/138533 (2008-11-01), None
Abdalrhman et al. 2006. Protection induced in mice against a lethal orthopox virus by the Lister strain of vaccinia virus and modified vaccinia virus Ankara (MVA). Vaccine 24(19):4152-4160.
Amosenko et al. 1991. Use of protamine sulphate for elimination of substrate DNA in poliovaccines produced on continuous cell lines. Vaccine 9(3):207-209.
Dancis. 1978. Shear breakage of DNA, Biophys. J. 24(2):489-503.
Deshmukh et al. 2005. Adsorptive purification of pDNA on superporous rigid cross-linked cellulose matrix. Journal of Chromatography B 818(1):5-10.
Diogo et al. 2001. Studies on the retention of plasmid DNA andEscherichia colinucleic acids by hydrophobic interaction chromatography. Bioseparation 10(4):211-220.
Diogo et al. 2005. Chromatography of plasmid DNA. Journal of Chromatography A 1069(1):3-22.
Diogo et al. 2000. Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies. Biotechnology Letters 22(17):1397-1400.
Esteban et al. 1973. Early virus protein synthesis in vaccinia virus-infected cells. J Gen Virol 19(2):201-6.
Freitas et al. 2009. Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase. Separation and Purification Technology 65(1):95-104.
Gagnon et al. 2006. Simultaneous Removal of Leached Protein A, Aggregates, DNA, and Endotoxins from MAbs. BioProcess International vol. 4, No. 2: pp. 50-60 4(2):50-60.
Gijsbers et al. 2005. Quantification of Residual Host Cell DNA in Adenoviral Vectors Produced on PER.C6® Cells. Hum. Gene Ther. 16(3):393-398.
Goerke et al. 2005. Development of a novel adenovirus purification process utilizing selective precipitation of cellular DNA. Biotechnology and Bioengineering 91(1):12-21.
Graumann et al. 2005. Development and Scale up of Preparative HIC for the Purification of a Recombinant Therapeutic Protein. Chemical Engineering & Technology 28(11):1398-1407.
Greenberg et al. 2008. ACAM2000: a newly licensed cell culture-based live vaccinia smallpox vaccine. Expert Opinion on Investigational Drugs 17(4):555-564.
Halder et al. 2005. Adsorption of biopolymers at hydrophilic cellulose-water interface. Biopolymers 77(5):286-295.
Iuliano et al. 2002. Rapid analysis of a plasmid by hydrophobic-interaction chromatography with a non-porous resin. Journal of Chromatography A 972(1):77-86.
Joklik. 1962. The preparation and characteristics of highly purified radioactively labelled poxvirus. Biochimica et Biophysica Acta (BBA)—Specialized Section on Nucleic Acids and Related Subjects 61(2):290-301.
Jordan et al. 2009. An avian cell line designed for production of highly attenuated viruses. Vaccine 27(5):748-756.
Kalbfuss et al. 2007. Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography. Biotechnology and Bioengineering 96(5):932-944.
Kato et al. 2004. Hydrophobic interaction chromatography at low salt concentration for the capture of monoclonal antibodies. Journal of Chromatography A 1036(1):45-50.
Knudsen et al. 2001. Membrane ion-exchange chromatography for process-scale antibody purification. Journal of Chromatography A 907(1-2):145-154.
Konz et al. 2005. Development of a Purification Process for Adenovirus: Controlling Virus Aggregation to Improve the Clearance of Host Cell DNA. Biotechnology Progress 21(2):466-472.
Kramarczyk et al. 2008. High-throughput screening of chromatographic separations: II. Hydrophobic interaction. Biotechnology and Bioengineering 100(4):707-720.
Kumar et al. 2002. Process standardization for optimal virus recovery and removal of substrate DNA and bovine serum proteins in Vero cell-derived rabies vaccine. Journal of Bioscience and Bioengineering 94(5):375-383.
Lu et al. 2009. Recent Advancement in Application of Hydrophobic Interaction Chromatography for Aggregate Removal in Industrial Purification Process Curr Pharm Biotechnol 10(4):427-433.
Madalinski et al. 1977. Purification of vaccinia virus by zonal centrifugation and analysis of viral protein composition. Acta virologica 21(2):104-108.
Mahn et al. 2005. Prediction of protein retention in hydrophobic interaction chromatography. Biotechnology Advances 23(5):359-368.
Monath et al. 2004. ACAM2000 clonal Vero cell culture vaccinia virus (New York City Board of Health strain)—a second-generation smallpox vaccine for biological defense. International Journal of Infectious Diseases 8(Supplement 2):31-44.
Opitz et al. 2009. Sulfated membrane adsorbers for economic pseudo-affinity capture of influenza virus particles. Biotechnol. and Bioeng. 103(6):1144-1154.
Opitz et al. 2007. Lectin-affinity chromatography for downstream processing of MDCK cell culture derived human influenza A viruses. Vaccine 25(5):939-947.
Opitz et al. 2008. Capture of cell culture-derived influenza virus by lectins: Strain independent, but host cell dependent. Journal of Virological Methods 154(1-2):61-68.
Påhlman et al. 1977. Hydrophobic interaction chromatography on uncharged sepharose® derivatives : Effects of neutral salts on the adsorption of proteins. Journal of Chromatography A 131:99-108.
Payne et al. 1976. Presence of Haemagglutinin in the Envelope of Extracellular Vaccinia Virus Particles. J Gen Virol 32(1):63-72.
Queiroz et al. 2001. Hydrophobic interaction chromatography of proteins. Journal of Biotechnology 87(2):143-159.
Sakata et al. 2007. Chromatographic Removal of DNA from Protein Solutions by Cationic Polymer Beads. Current Pharmaceutical Analysis 3:170-179.
Sakata et al. 2005. Chromatographic Removal of Host Cell DNA from Cellular Products Using Columns Packed with Cationic Copolymer Beads. Chromatographia 62(9):465-470.
Sheng-Fowler et al. 2009. Issues associated with residual cell-substrate DNA in viral vaccines. Biologicals 37(3):190-195.
Stickl et al. 1970. Purifying the vaccinia virus vaccine by gel filtration. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. 215(1):38-50.
Tauer et al. 1995. DNA clearance in chromatography of proteins, exemplified by affinity chromatography. Journal of Biochemical and Biophysical Methods 30(1):75-78.
Transfiguracion et al. 2003. Size-Exclusion Chromatography Purification of High-Titer Vesicular Stomatitis Virus G Glycoprotein-Pseudotyped Retrovectors for Cell and Gene Therapy Applications. Hum. Gene Ther. 14(12):1139-1153.
Triyoso et al. 1999. Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line. The Journal of Physiology 515(2):355-365.
Tsumoto et al. 2007. Arginine improves protein elution in hydrophobic interaction chromatography: The cases of human interleukin-6 and activin-A. Journal of Chromatography A 1154(1-2):81-86.
Ueberbacher et al. 2008. Hydrophobic interaction chromatography of proteins: V. Quantitative assessment of conformational changes. Journal of Chromatography A 1198-1199:154-163.
Wolff et al. 2008. Downstream Processing: From Egg to Cell Culture-Derived Influenza Virus Particles. Chemical Engineering & Technology 31(6):846-857.
Wolff et al. 2010. Capturing of cell culture-derived modified Vaccinia Ankara virus by ion exchange and pseudo-affinity membrane adsorbers, Biotechnology and Bioengineering Processing, 105(4):761-9 (Published online Nov. 4, 2009).
World-Health-Organization. 1998. WHO Expert Commitee on Biological Standardization. Worl Health Organ. Tech. Rep. Ser. 878(i-vi):1-101.
Zwartou
Faber Rene
Gram Anders Peter
Post Hansen Sara
Reichl Udo
Wolff Michael
Bavarian Nordic A/S
Law Office of Salvatore Arrigo and Scott Lee, LLP
Mosher Mary E
Otto-von-Guericke-Universität
Sartorius Stedim Biotech GmbH
LandOfFree
Purification of vaccinia viruses using hydrophobic... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of vaccinia viruses using hydrophobic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of vaccinia viruses using hydrophobic... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2749337