Purification of vaccinia virus- and recombinant vaccinia...

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification

Reexamination Certificate

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C424S199100, C424S232100

Reexamination Certificate

active

08003363

ABSTRACT:
The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.

REFERENCES:
patent: 6267967 (2001-07-01), Johnston et al.
patent: 2003/0165477 (2003-09-01), Balloul
patent: 2007/0071769 (2007-03-01), Sutter
patent: WO03/054175 (2003-07-01), None
O'Keeffe et al (Biotechnology and Bioengineering 62:537-545, 1999).
Chung et al (Journal of Virology 72:1577-1585, 1998).
Baba et al., Sulfated Polysaccharides Are Potent and Selective Inhibitors of Various Enveloped Viruses, Including Herpes Simplex Virus, Cytomegalovirus, Vesicular Stomatitis Virus, and Human Immunodeficiency Virus, Antimicrobial Agents and Chemotherapy 32 (1988) 1742-1745.
Chattopadhyay et al., Polysaccharides fromGracilaria corticata: Sulfation, chemical characterization and anti-HSV activities, International Journal of Biological Macromolecules 43 (2008) 346-351.
Enden et al., A model of the dynamics of insect cell infection at lowmultiplicity of infection, Journal of Theoretical Biology 237 (2005) 257-264.
Ho et al., The Oligomeric Structure of Vaccinia Viral Envelope Protein A27L is Essential for Binding to Heparin and Heparan Sulfates on Cell Surfaces: A Structural and Functional Approach Using Site-specific Mutagenesis, J. Mol. Biol. (2005) 349, 1060-1071.
Hsaio et al., Cell Surface Proteoglycans Are Necessary for A27L Protein-Mediated Cell Fusion: Identification of the N-Terminal Region of A27L Protein as the Glycosaminoglycan-Binding Domain, Journal of Virology 72 (1998) 8374-8379.
Hsaio et al., Vaccinia Virus Envelope D8L Protein Binds to Cell Surface Chondroitin Sulfate and Mediates the Adsorption of Intracellular Mature Virions to Cells, Journal of Virology 73 (1999) 8750-8761.
Kalbfuss et al., Purification of Cell Culture-Derived Human Influenza A Virus by Size-Exclusion and Anion-Exchange Chromatography, Biotechnology and Bioengineering 96 (2007) 932-944.
Lin et al., Vaccinia Virus Envelope H3L Protein Binds to Cell Surface Heparan Sulfate and Is Important for Intracellular Mature Virion Morphogenesis and Virus Infection In Vitro and In Vivo, Journal of Virology 74 (2000) 3353-3365.
Lycke et al., Binding of herpes simplex virus to cellular heparan sulphate, an initial step in the adsorption process, Journal of General Virology (1991) 72, 1131-1137.
Mitsuya et al., Dextran Sulfate Suppression of Viruses in the HIV Family: Inhibition of Virion Binding to CD4+ Cells, Science 240 (1988) 646-649.
O'Keeffe et al., The Affinity Adsorptive Recovery of an Infectious Herpes Simplex Virus Vaccine, Biotechnology and Bioengineering 62 (1999) 537-545.
Opitz et al., Lectin-affinity chromatography for downstream processing of MDCK cell culture derived human influenza A viruses, Vaccine 25 (2007) 939-947.
Opitz et al., Sulfated Membrane Adsorbers for Economic Pseudo-Affinity Capture of Influenza Virus Particles, Biotechnology and Bioengineering 103 (2009) 1144-1154.
Peixoto et al., Towards Purification of Adenoviral Vectors Based on Membrane Technology, Biotechnol. Prog. 2008, 24, 1290-1296.
Piret et al., In Vitro and In Vivo Evaluations of Sodium Lauryl Sulfate and Dextran Sulfate as Microbicides against Herpes Simplex and Human Immunodeficiency Viruses, Journal of Clinical Microbiology 38 (2000) 110-119.
Resch et al., Protein composition of the vaccinia virus mature virion, Virology 358 (2007) 233-247.
Smith et al., The formation and function of extracellular enveloped vaccinia virus, Journal of General Virology (2002) 83, 2915-2931.
Wu et al., Ion-Exchange Membrane Chromatography Method for Rapid and Efficient Purification of Recombinant Baculovirus and Baculovirus gp64 Protein, Human Gene Therapy (2007) 18:665-672.
Harrer et al., Therapeutic vaccination of HIV-1-infected patients on HAART with a recombinant HIV-1 nef-expressing MVA: safety, immunogenicity and influence on viral load during treatment interruption, Antiviral Therapy 10:285-300 (2005).
Shen et al., Fighting Cancer with Vaccinia Virus: Teaching New Tricks to an Old Dog, Molecular Therapy 11:180-195 (2005).
Chung et al., A27L Protein Mediates Vaccinia Virus Interaction with Cell Surface Heparan Sulfate, Journal of Virology 72:1577-1585 (1998).
Zahn et al., Hepatitis C virus and hepatitis B virus bind to heparin: purification of largely IgG-free virions from infected plasma by heparin chromatography, J. Gen. Virology 86:677-685 (2005).
Broder et al., Recombinant Vaccinia Viruses, Molecular Biotechnology 13:223-245 (1999).
Wolff et al., Capturing of Cell Culture-Derived Modified Vaccinia Ankara Virus by Ion Exchange and Pseudo-Affinity Membrane Adsorbers, Biotechnology and Bioengineering 105: 761-769 (2010) [e-pub Nov. 4, 2009].
O'Neil et al., Virus Harvesting and Affinity-based Liquid Chromatograpy, Bio/Technology vol. 11:173-178 (1993).

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