Purification of urokinase

Details Purification of urokinase Purification of urokinase

1975-11-05

1978-08-15

Naff, David M.

Textiles: fiber preparation

195 63, 195 66R, 195 68, 424 94, C07G 702

Patent

active

041069927

ABSTRACT:
An initial solution of crude urokinase especially of human origin, is subjected to exclusion chromatography by contact with a DEAE cellulose resin, following adjustment of the pH of the solution to a value of from 4 to 6, and of its conductivity to a value of from 15,000 to 25,000 micromhos. An effluent enriched in urokinase is collected. A urokinase, mixed with foreign proteins, especially pyrogens, is purified by a partial saturation of an aqueous solution of this mixture by ammonium sulphate to a value for which the precipitate formed would not entrain more than 5% of the total activity of urokinase of the initial solution. A supernatant liquid enriched in urokinase is collected . A complex of urokinase and heparin, urokinase heparinate, is made by reacting an enriched solution of urokinase with a solution of heparin. It has relative activities of 30,000 to 100,000 CTA units of urokinase for 100 Iu of heparin. In an isotonic perfusion solution in glucose serum, its dilution is from 300,000 to 900,000 CTA units of urokinase/250 ml.

REFERENCES:
patent: 3477912 (1969-11-01), Sloane
patent: 3755083 (1973-08-01), Novak
Dixon et al., Enzymes, Academic Press Inc., N.Y., 1958, (pp. 47-50).

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