Purification of uricase

Textiles: fiber preparation

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195 66R, 1951035R, C12D 1310

Patent

active

040640108

ABSTRACT:
Catalase is effectively removed from a uricase preparation by amphiphilic chromotography using as the column material cyanogen bromide-activated polysaccharide having a hydrophobic ligand attached thereto. Particularly useful hydrophobic ligands are the alkanediamines.

REFERENCES:
patent: 3431176 (1969-03-01), Fukumoto et al.
patent: 3475276 (1969-10-01), Kano
patent: 3620923 (1971-11-01), Laboureur et al.
patent: 3669843 (1972-06-01), Aunstrup et al.
patent: 3767533 (1973-10-01), Sugisaki et al.
patent: 3810820 (1974-05-01), Laboureur et al.
Cuatrecasas, "Affinity Chromatography of Macromolecules", in Advances in Enzymology, vol. 36, pp. 29-89, 1972.

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