Purification of tuberculoproteins

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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424 92, C07G 700

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040425746

ABSTRACT:
An aqueous solution in a neutral medium of the tuberculoprotein C is subjected to the action of an ionized substance before carrying out three different separation processes which consist in bringing the tuberculoprotein C into solution in water in a proportion of 5% by weight and in neutralizing. The first process consists in adding the ionized substance in an alcohol medium and in carrying out ultracentrifugation for two hours at 40,000 g in order to obtain tuberculoproteins CA in the form of a supernatant liquid. The second process consists in adding a solution of the ionized substance, in carrying out ultrafiltration, concentration and precipitation with trichloroacetic acid in order to obtain the tuberculoprotein CB. The third process consists in adding a solution of the ionized substance then gel-filtering in order to eliminate the molecules having a molecular weight above 200,000 g and in precipitating with trichloroacetic acid in order to obtain a tuberculoprotein CC.

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