Purification of tissue plasminogen activator (tPA)

Details Purification of tissue plasminogen activator (tPA) Purification of tissue plasminogen activator (tPA)

2006-07-11

2006-07-11

Ponnaluri, Padmashri (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S320100, C435S069100, C435S091500, C536S023200, C536S023400, C536S023500

Reexamination Certificate

active

07074560

ABSTRACT:
A method is disclosed for obtaining affinity ligands for isolating tissue-type plasminogen activator (tPA). Ligands binding tPA with high specificity at pH 7 and releasing tPA at pH 5 or lower are disclosed. Also disclosed are methods whereby additional ligands having desirable preselected binding and release (elution) characteristics may be isolated, permitting the development of tailored ligands to meet the purification problems presented by any particular feed stream containing tPA.

REFERENCES:
patent: 4708944 (1987-11-01), Someno et al.
patent: 4898825 (1990-02-01), Morii et al.
patent: 5133866 (1992-07-01), Kauvar
patent: 5141862 (1992-08-01), Patel et al.
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5227297 (1993-07-01), Patel et al.
patent: 5403484 (1995-04-01), Ladner et al.
patent: 6084062 (2000-07-01), Maclennan et al.
patent: 0 112 122 (1984-06-01), None
Bode et al.,The refined 2.0 angstrom X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds(Cucurbita maxima).Febs Letters242 (2):285-292.
Boschetti,Advanced sorbents for preparative protein separation purposes, J. Chromatography A, 658: 207-236 (1994).
Garrard & Henner, Gene 128: 103-109 (1993).
Huang et al.,Affinity purification of von Willebrand factor using ligands derived from peptide libraries, Bioorganic&Medicinal Chem., 4(5): 699-708 (1996).
Knight,Bioseparations: media and modes, Bio/Technology, 8: 200-201 (1990).
Ladner,Constrained peptides as binding entities, Trends in Biotechnology, 13(10): 426-430 (1995).
Le Nguyen et al,Solid phase synthesis of a trypsin inhibitor isolated from the Cucurbitaceae Ecballium elaterium, Int. J. Peptide Protein Res., 34: 492-497 (1989).
Markland et al.,Selection for protease inhibitors using bacteriophage display, Methods Enzymol., 267: 28-51 (1996).
Narayanan,Preparative affinity chromatography of proteins, J. Chromatography A, 658: 237-258 (1994).
Scopes,Protein Purification: Principles and Practice, New York, Springer-Verlag, 111-112, 117-125 (1982).
Vedvick et al.,High-level secretion of biologically active aprotinin from the yeast Pichia pastoris, J. Industrial Microbiol., 7: 197-202 (1991).
Wagner et al.,High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor, Biochem. Biophys Res. Comm., 186(2): 1138-1145 (1992).
Weiczorek et al.,The squash family of serine proteinase inhibitors, BBRC, 126:646-652 (1985).
Kupryszewski et al., “Synthesis of Trypsin Inhibitor CMTI I from Squash Seeds,”Polish J. of Chem., 1987, vol. 61, pp. 789-793.
PCT International Search Report, mailed Jul. 8, 1998.

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