Purification of the messenger RNA cap-binding protein

Details Purification of the messenger RNA cap-binding protein Purification of the messenger RNA cap-binding protein

1984-12-27

1987-12-01

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 29, 530827, C07H 1906, C07H 1910, C07H 1916

Patent

active

047105666

ABSTRACT:
The synthesis of a new affinity medium, the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate is described.

REFERENCES:
patent: 4012570 (1977-03-01), Dean et al.
patent: 4322410 (1982-03-01), Stjepanovic et al.
patent: 4401759 (1983-08-01), Rubin et al.
patent: 4500707 (1985-02-01), Caruthers et al.

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