Purification of T-cell growth factor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530412, 530417, 530416, 435 68, C07K 320, C07K 328

Patent

active

046753834

ABSTRACT:
Substantially homogenous T-cell growth factor is isolated. A multiple-step purification procedure of T-cell growth factor-containing biological sample includes at least one reverse-phase HPLC fractionation using a C3 to C5 silica gel stationary phase. A preferred pre-purification protocol includes concentration of the biological sample on a semipermeable medium followed by gel filtration and semi-preparative reverse-phase HPLC on a hydrophobic stationary phase. Fractionating the pre-purified biological sample with two or three reverse-phase HPLC fractionations using C3 to C5 silica gel stationary phase yields T-cells growth factor of over 90% purity.

REFERENCES:
patent: 4289690 (1981-09-01), Pestka et al.
patent: 4377482 (1983-03-01), Rivier
patent: 4401756 (1983-08-01), Gillis
patent: 4406830 (1983-09-01), Fabricius et al.
patent: 4490289 (1984-12-01), Stern
Gillis et al., Molecular Characterization of IL-2, Immunol. Rev. 63, pp. 167-209 (1982).
Robb et al., Proc. Natl. Acad. Sci. USA. 80, pp. 5990-5994 (1983).
Taniguchi et al., Nature, vol. 302, pp. 305-310 (1983).
Milestone et al., Biochem. Biophy. Res. Comm., vol. 115, pp. 762-768 (1983).
Anzano et al., Analytical Biochemistry 125, pp. 217-224 (1982).
Fuhrer et al., J. Chromato 248(3), pp. 427-433 (1982).
FASEB 1982, Lymphokine Res., vol. (2), pp. 51-57 (1982).
Ihle et al., J. Immunol. 129 (6), pp. 2431-2436 (1982).
Mahoney, Biochem. Biophy. Acta. 704, pp. 284-289 (1982).
Purification of Mouse IL-2 to Apparent Homogeneity, JBC 258(20) 1983, pp. 12114-12117, Riendeau et al.
A Rapid Large Scale Purification Procedure for Gibbon IL-2, J. Immunol. 131(2) 1983, pp. 810-815, Henderson.
Control of the Expression of IL-2 Activity Cell Immunol. 73, pp. 106-114 (1982) Henriksen et al.
HPLC and Its Application to Protein Chemistry Advances Chromatography, (NY) (1981) Chapter 1, pp. 1-84 Hearn.
High-Performance Liquid Chromatography of Proteins, Analytical Biochemistry 103, pp. 1-25 (1980) Regaier et al.
Quantitative Determination of Amino Acids . . . HPLC, Biochem. Biophys. Res. Comm., 108(2), pp. 783-790 (1982) Rabus et al.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Purification of T-cell growth factor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of T-cell growth factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of T-cell growth factor will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-721870

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.