Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1990-04-27
1993-10-26
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
935 88, 530334, 530335, 530336, 530337, 530344, 536 253, 536 2531, 435 915, 435 913, C12P 1934, C07K 104, C07K 106, C07K 114
Patent
active
052565491
ABSTRACT:
Oligomers and polymers are prepared substantially free of error sequences by sequentially adding monomers, which are terminally blocked and have active functionalities protected, to a growing chain bound to a support through a selectively cleavable linkage. After each addition, unblocked terminal groups are capped. At the completion of monomer addition, enzymatic hydrolysis interfering protecting groups are removed along with the capping group and failure sequences enzymatically degraded. The terminal blocking group may then be removed. The completed oligomer or polymer may be cleaved from the support prior or subsequent to enzymatic degradation but after completion of the sequence.
REFERENCES:
patent: 4483964 (1984-11-01), Urdea et al.
patent: 4517338 (1985-05-01), Urdea et al.
patent: 4521509 (1985-01-01), Benkovic et al.
Phillippsen et al., "Splitting of Phenylalanine Specific tRNA into Half-Molecules by Chemical Means", Biochem Biophys Res. Comm. vol. 33, 922-928, 1968.
McBride et al., "Amidine Protecting Groups for Oligonucleotide Synthesis" J. Am. Chem. Soc., vol. 108, 2040-2048, 1986.
Horn Thomas
Urdea Michael S.
Blackburn Robert P.
Chiron Corporation
Elliott George C.
Goldman Kenneth M.
Reed Dianne E.
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