Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1990-05-01
1994-08-30
Hollrah, Glennon H.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
549332, 549510, 549511, C07D32100, C07F 912
Patent
active
053429664
ABSTRACT:
Methods are disclosed for purifying chemiluminescent water-soluble 1,2-dioxetane derivatives suitable for use as reporter molecules in a variety of biological analytical systems, including enzyme-linked immunoassays, nucleic acid probe techniques, and structural determinations. The methods are based upon high pressure, medium pressure or low pressure liquid chromatography using as the stationary phase alkaline pH-stable compositions with the chromatographic characteristics of reversed-phase adsorbents, at alkaline pH values, and in the absence of acid-forming compounds or compounds with an unshared pair of electrons. Desalting of substantially pure water soluble 1,2-dioxetane derivatives may be accomplished by the same chromatographic systems or by molecular sieve chromatography systems, but in the absence of salt buffers. Under some circumstances, purification and desalting may be combined in a single chromatographic step. Substantially pure chemiluminescent water-soluble 1,2-dioxetane derivatives prepared by the methods of the invention are also disclosed.
REFERENCES:
patent: 5089630 (1992-02-01), Bronstein et al.
Jefford, C. W. et al. "Determination Of The Thermal Stability Of Some 1,2-dioxetanes By High Performance Liquid Chromatography" Journal Of Chromatography, 347 (1985) 183-187.
Adam et al. "Synthesis Thermal Stability And Chemiluminescence Properties Of The Dioxetanes Derived From 1,4-Dioxins" J. Org. Chem. 1984, 49, 3920-3928.
Baumstark et al. "Thermolysis Of 3-Methyl-3-Alkyl-1,2-Dioxetanes: Steric Effects On The Activation Parameters" J. Org. Chem. 1983, 48, 3713-3716.
Vogel, Arthur I. A Textbook Of Practical Organic Chemistry Including Qualitative Organic Analysis, London, Longmans, Green & Co. pp. 160-161 (1948).
Becker et al. Organicum Practical Handbook Of Organic Chemistry, Oxford Pergamon Press Ltd. 1973, pp. 78-82.
Edwards Brooks
Voyta John C.
Hollrah Glennon H.
Pak John D.
Tropix, Inc.
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