Purification of somatotropin from transformed microorganisms

Details Purification of somatotropin from transformed microorganisms Purification of somatotropin from transformed microorganisms

2005-07-12

2005-07-12

Yucel, Remy (Department: 1636)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C530S350000, C530S351000, C530S399000, C435S069400

Reexamination Certificate

active

06916914

ABSTRACT:
This invention discloses a commercially advantageous process for extraction and purification of protein from microorganisms. The initial steps of the process are useful for purifying many insoluble proteins while later steps are designed to renature denatured somatotropins produced by transformed microorganisms. The process is especially useful for purifying recombinantly-produced bovine somatotropin.

REFERENCES:
patent: 4443539 (1984-04-01), Fraser et al.
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olson
patent: 4599197 (1986-07-01), Wetzel
patent: 4612367 (1986-09-01), Grinnan et al.
patent: 4620948 (1986-11-01), Builder et al.
patent: 4652630 (1987-03-01), Bentle et al.
patent: 47 600 (1981-08-01), None
patent: 103 395 (1983-08-01), None
patent: 114 506 (1983-12-01), None
patent: 131 843 (1984-07-01), None
patent: 2073245 (1981-03-01), None
patent: 2073245 (1981-10-01), None
Wetzel etal. Biochemistry 19 6096-6104 1980.
D.V. Goeddel et al., Direct Expression inEscherichia coliof a DNA Sequence Coding for Human Growth Hormone, Nature 281:544-548 (1979).
A.C. Paladini et al., Molecular Biology of Growth Hormone, CRC Reviews in Biochem., 15(1):25-56 (1983).
P.J. Eppard and D.E. Bauman, The Effect of Long-Term Administration of Growth Hormone on Performance of Lactating Dairy Cows, Proc. 1984 Cornell Nutrition Conf. for Feed Manufacturuers, pp. 5-17.
B.E. Schoner et al., Role of mRNA translational efficiency in bovine growth hormone expression inEscherichia coli, PNAS, USA, 81:5403-5407 (1984).
P.H. Seeburg et al., Efficient Bacterial Expression of Bovine and Porcine Growth Hormones, DNA, 2(1):37-45 (1983).
G. Gray et al., Synthesis of bovine growth hormone by Streptomyces lividans, Gene 32:21-30 (1984).
R.G. Schoner et al., Isolation and Purification of Protein Granules fromEscherichia coliCells Overproducing Bovine Growth Hormone, Bio/Tech., 3:151-154 (1985).
M. Teuber, Archiv fur Mikrobiologie 55:31-45 (1966); Chem. Abstracts, Abstract 16597y, 66(5): 1593-1954, Jan. 30, 1967.
A.M. Plommet et al., Ann. Rech. veter., 5(2):213-221 (1974); Chem. Abstracts, Abstract 100544w, 81(17):138, Oct. 28, 1974.
B.D. Davis et al., Inactivation of Viruses, Microbiology, 3rd edition, Harper & Row, New York (US), pp. 1271-1274 (1980).

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