Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1996-06-10
1998-06-02
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530414, 530416, 530417, 530418, 530427, 4352401, 4352402, 43524021, 435240243, C07K 100, C12N 500
Patent
active
057601798
ABSTRACT:
A method for purifying soluble laminin 5 from conditioned cell culture medium. A nonionic or anionic detergent is added to conditioned medium to a final concentration of between 0.1% and 1.0%. The conditioned medium is purified by cation exchange chromatography and anion exchange chromatography, yielding laminin 5 of at least about 70% purity.
REFERENCES:
patent: 5422264 (1995-06-01), Quaranata et al.
patent: 5510263 (1996-04-01), Quaranta et al.
patent: 5541106 (1996-07-01), Jones
Baker et al, Experimental Cell Research, vol. 228, No. 2, pp. 262-270, Feb. 7, 1996.
Miyazaki et al, Proc. Natl. Acad. Sci, USA, vol. 90, pp. 11767-11771, 1993.
Rousselle et al, The Journal of Biological Chemistry, vol. 270, No. 23, pp. 13766-13770, Jun. 9, 1995.
Chan et al, J. Invest. Dermatol., vol. 105, pp. 75-79, 1995.
W.G. Carter, et al. (1991) "Epiligrin, A New Cell Adhesion Ligand for Integrin .alpha.3.alpha.1 in Epithelial Basement Membranes", Cell, 65:599-610.
B. Hsi, et al. (1987) "Monoclonal Antibody GB.sub.3 6 Raised Against Human Trophoblast Recognizes a Novel Epithelial Antigen", Placenta, 8:209-217.
K. Izumi, et al. (1981) "In Vitro Induction of Ornithine Decarboxylase in Urinary Bladder Carcinoma Cells", Cancer Research, 41:405-409.
K. Miyazaki, et al. (1993) "A Large Cell-Adhesive Scatter Factor Secreted by Human Gastric Carcinoma Cells", Proc. Natl. Acad. Sci. USA, 90:11767-11771.
P. Rousselle, et al. (1991) "Kalinin: An Epithelium-Specific Basement Membrane Adhesion Molecule That is a Component of Anchoring Filaments", Journal of Cell Biology, 114(3):567-576.
H.D. Soule, et al. (1990) "Isolation and Characterization of a Spontaneously Immortalized Human Breast Epithelial Cell Line, MCF-10", Cancer Research, 50:6075-6086.
L.A. Staehelin (1974) "Structure and Function of Intercellular Junctions". Department of Molecular. Cellular and Developmental Biology. University of Colorado, Boulder, Colorado, pp. 191-283.
L. Tait, et al. (1990) "Ultrastructural and Immunocytochemical Characterization of an Immortalized Human Breast Epithelial Cell Line, MCF-10", Cancer Research, 50:6087-6094.
P. Verrando, et al. (1988) "The New Basement Membrane Antigen Recognized by the Monoclonal Antibody GB3 is a Large Size Glycorprotein: Modulation of its Expression by Retinoic Acid", Biochimica et Biophysica Acta. 942:45-56 .
Desmos, Inc.
Mohamed Abdel A.
Tsang Cecilia J.
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