Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1986-12-23
1990-01-16
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
530351, 424 856, C12P 2100, A61K 4502, C07K 1526
Patent
active
048943304
ABSTRACT:
Improved RP-HPLC methods for purifying recombinant betainterferon are disclosed. Said RP-HPLC methods employ wide pore silica gel reverse-phase columns and solvent systems containing acetonitrile as the organic modifier and either heptafluorobutyric acid or trifluoroacetic acid as the organic acid.
The invention further concerns processes for purifying recombinant IFN-.beta. incorporating said improved RP-HPLC methods.
The invention further concerns recombinant IFN-.beta. purified by said RP-HPLC methods, or recovered and/or purified by processes incorporating said RP-HPLC.
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Hershenson Susan
Shaked Ze'Ev
Cetus Corporation
Halluin Albert P.
Hasak Janet E.
Hazel Blondel
McGarrigle Philip L.
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