Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1979-08-31
1981-06-09
Fagelson, Anna P.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 250302, 260112R, 260112B, 424 1, 424 12, 424 13, 424 85, 424 88, 424177, 424180, 435 4, 435 7, C07G 700, C12Q 100, G09K 1107, G01N 3352
Patent
active
042725062
ABSTRACT:
A method is provided for preparing immunoassay reagents involving labeled members of specific binding pairs substantially enriched relative to contaminating labeled materials. The method involves conjugating a member of a specific binding pair to a support by a covalent bond which is cleavable under mild conditions to provide a binding pair member-support conjugate. Combining the binding pair member-support conjugate with a labeled composition containing the reciprocal member of the binding pair, so that the labeled reciprocal member becomes bound to the support through the binding of the specific binding pair. Separating the support to which is bound the labeled member from the remaining labeled material and then cleaving the bond joining the labeled specific binding pair to the support to provide labeled reagent for immunoassays. In particular, an antibody is linked to a support by disulfide linkage and a composition containing the reciprocal antigen to the antibody is labeled with a chromophore, particularly fluorescer. The support is freed of labeled material other than the desired labeled antigen and the disulfide link cleaved to provide labeled reagent for immunoassays.
REFERENCES:
patent: 3652761 (1972-03-01), Weetall
patent: 3690834 (1972-09-01), Goldstein
patent: 3998943 (1976-12-01), Ullman
patent: 4039652 (1977-08-01), Adams
patent: 4160016 (1979-07-01), Ullman
patent: 4176006 (1979-11-01), Cormier
Fagelson Anna P.
Rowland Bertram I.
Syva Company
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