Purification of Q Beta replicase

Details Purification of Q Beta replicase Purification of Q Beta replicase

1989-06-09

1992-08-25

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

424 945, 435194, 435220, C12D 1934

Patent

active

051418576

ABSTRACT:
A process of producing highly pure, Q Beta replicase having a high level of activity is described. The present process allows isolation of Q Beta replicase from recombinant bacteria containing a clone of a phage DNA encoding the 65,000 weight subunit of Q Beta replicase. The present process provides an efficient method for producing pure Q Beta replicase which can readily be scaled up to commercial production levels.

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