Purification of proteins employing CTAP-III fusions

Details Purification of proteins employing CTAP-III fusions Purification of proteins employing CTAP-III fusions

1989-05-04

1993-01-12

Patterson, Jr., Charles L.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530412, 530415, 530416, 530417, 435 697, C07K 322, C07K 1300

Patent

active

051791960

ABSTRACT:
The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.

REFERENCES:
patent: 3432596 (1969-03-01), Markwardt
patent: 4668662 (1987-05-01), Tripier
patent: 4745177 (1989-05-01), Fritz et al.
patent: 4767742 (1988-08-01), Dodt et al.
patent: 4897348 (1990-01-01), Johnson et al.
Pharmacia, 1980, "Ion Exchange Chromatography: Principles and Methods", Rahmr i Lund, Sweden, pp. 29-33.
Ochoa, J. L., 1978, Biochimie, 60: 1-15.
Sofer et al., 1983, Bio Technique, Nov./Dec., pp. 198-203.
Pharmaceuticals, p. 14, Mar. 1986, English Abstract (corresponding to EP 168,342).
Pharmaceuticals, pp. 5-6, week 8607, English Abstract (corresponding to EP 171,024).
Pharmaceuticals, p. 9, week 8636, English Abstract (corresponding to EP 193,175).
Pharmaceuticals, p. 6, English Abstract, (corresponding to EP 209,061).
Pharmaceuticals, p. 6, week 8646, English Abstract, (corresponding to EP 200,655).
Harvey et al., (1986), Proc. Natl. Acad. Sci. U.S.A. 83: 1084-1088.
Dodt et al., (1986) FEBS 3830 202(2): 373-377.
Fortkamp et al. (1986) DNA 5(6): 511-517.
Chang (1983) FEBS 1044 164(2): 307-313.
Baskova et al., (1983) Thromb Res 30: 459-467.
Markwardt (1985) Biomed Biochim Acta 44: 1007-1013.
Green et al., (1989) Mol Cell Biol 9(4): 1445-1451.
Waleh & Johnson (1985) Proc. Natl. Acad. Sci. U.S.A. 82: 8389-8393.

Affiliated with

Also associated with

Rating

Purification of proteins employing CTAP-III fusions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of proteins employing CTAP-III fusions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of proteins employing CTAP-III fusions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1221180