Purification of proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530364, 210660, 210668, 435 711, 435 712, C07K1/18

Patent

active

059051434

ABSTRACT:
When proteins are purified using a protein-binding dye immobilized on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. An ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein to overcome the protein of the leaking dye.

REFERENCES:
patent: 4195126 (1980-03-01), Hall
patent: 4374930 (1983-02-01), Snoke et al.
patent: 4623625 (1986-11-01), Scopes
patent: 4740306 (1988-04-01), Litwack et al.
patent: 4990447 (1991-02-01), Konig et al.
patent: 5136026 (1992-08-01), Romisch et al.
patent: 5294699 (1994-03-01), Ohmura
patent: 5625041 (1997-04-01), Johnson et al.
Jap. Soc. Biochem. "The Protein I-Separation, Purification and Properties", Tokyo Kakagu Dojin, 216 & 229 (Feb. 1990).
Harris, et al, ed., "Purification Methods", IRL Press (1989), pp. 245-246, 252-254, 258-261.
Travis et al, Biochem J. vol. 157, (1976) pp. 301-306.
Lascu et al, J. of Chromatography, vol. 283, (1984) pp. 199-210.
Bernabeu et al, Eur. J. of Biochem., vol. 189 (1980) pp. 285-290.
Wichman et al, Biochimica et Biophbica aeta, vol. 273 (1974) pp. 218-224.
Kyoritsu Shuppan "Extra Number: Protein, Nucleic Acid and Enzyme--Affinity Chromatography & Affinity Label", Kunihiko Ishikawa (ed.), (Mar. 1, 1980), pp. 66-71.
Scopes, Protein Purification, Principles and Practice (Springer Verlag. NY, 2nd Editio pp. 141-157), 1988.
Van Damme et al, J. Chromatog, vol. 57, pp. 158-160, 1971.
King, Transfusion, vol. 27, pp. 302-308, 1987.
Chem.Abs. 94127700v., 1981.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Purification of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1760143

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.